LOCUS       sequence-86020-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 
            with GyrA intein linker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Weber AR, Schuermann D, Schar P
  TITLE     Versatile Recombinant SUMOylation System for the Production of 
            SUMO-Modified Protein.
  JOURNAL   PLoS One. 2014 Jul 9;9(7):e102157. doi: 
            10.1371/journal.pone.0102157. eCollection 2014.
  PUBMED    25007328
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-9"; volume: "9(7):e102157. doi"; pages: " 
            10.1371/journal.pone.0102157. eCollection 2014"
COMMENT     Sequence Label: pSC-IntE2
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             82..675
                     /codon_start=1
                     /gene="Mycobacterium xenopi gyrA"
                     /product="modified GyrA intein (Southworth et al., 1999)"
                     /label=Mxe GyrA intein
                     /note="N-terminal cleavage of this intein can be induced by
                     thiols."
                     /translation="CICGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPV
                     LADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAV
                     IQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYY
                     AKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHA"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataaccatgg ctagctcgcg agtcgacggc
       61 ggccgcgaat tcctcgagat gtgcatctgc ggagatgcac tagttgccct acccgagggc
      121 gagtcggtac gcatcgccga catcgtgccg ggtgcgcggc ccaacagtga caacgccatc
      181 gacctgaaag tccttgaccg gcatggcaat cccgtgctcg ccgaccggct gttccactcc
      241 ggcgagcatc cggtgtacac ggtgcgtacg gtcgaaggtc tgcgtgtgac gggcaccgcg
      301 aaccacccgt tgttgtgttt ggtcgacgtc gccggggtgc cgaccctgct gtggaagctg
      361 atcgacgaaa tcaagccggg cgattacgcg gtgattcaac gcagcgcatt cagcgtcgac
      421 tgtgcaggtt ttgcccgcgg gaaacccgaa tttgcgccca caacctacac agtcggcgtc
      481 cctggactgg tgcgtttctt ggaagcacac caccgagacc cggacgccca agctatcgcc
      541 gacgagctga ccgacgggcg gttctactac gcgaaagtcg ccagtgtcac cgacgccggc
      601 gtgcagccgg tgtatagcct tcgtgtcgac acggcagacc acgcgtttat cacgaacggg
      661 ttcgtcagcc acgctactgg cctcaccggt ctaagcttag catccatgtc ggggatcgcc
      721 ctcagcagac tcgcccagga gaggaaagca tggaggaaag accacccatt tggtttcgtg
      781 gctgtcccaa caaaaaatcc cgatggcacg atgaacctca tgaactggga gtgcgccatt
      841 ccgggaaaga aagggactcc gtgggaagga ggcttgttta a
//
