LOCUS       sequence-86029-e         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of mouse TDG-K341R C-terminally fused to GyrA 
            intein, SUMO-E2 and GST.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Weber AR, Schuermann D, Schar P
  TITLE     Versatile Recombinant SUMOylation System for the Production of 
            SUMO-Modified Protein.
  JOURNAL   PLoS One. 2014 Jul 9;9(7):e102157. doi: 
            10.1371/journal.pone.0102157. eCollection 2014.
  PUBMED    25007328
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-9"; volume: "9(7):e102157. doi"; pages: " 
            10.1371/journal.pone.0102157. eCollection 2014"
COMMENT     Sequence Label: pSC-mTDG-K341R-IntE2
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataaccatgg acgcagaggc cgcgcgcagc
       61 tattctctgg agcaagttca agctttgtat tcatttccat ttcaacaaat gatggcagaa
      121 gttcctaaca tggcagtcac gactggacag caggtgccag cagtagctcc taacatggca
      181 accgtgacag aacagcaggt gcccgcagac gctcctgtcc aggaacctgc accagaagct
      241 ccaaagagaa ggaaaaggaa acccagagca gcagagcccc aggaaccagt ggagcccaaa
      301 aaacctgcta cgtcgaagaa atccggcaag tctacaaaat caaaggaaaa gcaggagaaa
      361 atcacagacg cgtttaaagt gaaaaggaaa gtggaccgct tcaacggcgt ctctgaagct
      421 gagcttctga ccaagactct tcctgacatt ttgaccttca atctggatat tgtgatcatt
      481 ggcattaacc cgggattaat ggctgcttac aaaggacatc actaccctgg gcctggaaat
      541 cacttctgga agtgtctgtt catgtcgggg ctgagtgagg tgcagctgaa tcacatggat
      601 gaccacacct tacccggcaa gtacggcatc ggattcacca acatggtgga acggacgacg
      661 ccgggcagca aggatctgtc tagtaaagag ttccgggaag gagggcgcat cctggtgcag
      721 aaactgcaga aatatcagcc acgaatagcg gtgtttaatg gaaaatgtat ttatgaaatt
      781 ttcagtaaag aagtttttgg agtaa
//
