LOCUS       sequence-86030-e         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To create stable cell clones with high-level expression of Cas9 and 
            OCT1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Zhou Y, Zhu S, Cai C, Yuan P, Li C, Huang Y, Wei W
  TITLE     High-throughput screening of a CRISPR/Cas9 library for functional 
            genomics in human cells.
  JOURNAL   Nature. 2014 Apr 9. doi: 10.1038/nature13166.
  PUBMED    24717434
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Apr 9. doi: 10.1038/nature13166."
COMMENT     Sequence Label: pLenti-OC-IRES-BSD
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..25
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
       61 tagaagacac cgactctaga ggatccggac tagtttaccg gtgggggccc ccggcgcgcc
      121 ggtgtacacc ctgcaggggt ttatgaacaa tccgtcagaa accagtaaac catctatgga
      181 gagtggagat ggcaacacag gcacacaaac caatggtctg gactttcaga agcagcctgt
      241 gcctgtagga ggagcaatct caacagccca ggcgcaggct ttccttggac atctccatca
      301 ggtccaactc gctggaacaa gtttacaggc tgctgctcag tctttaaatg tacagtctaa
      361 atctaatgaa gaatcggggg attcgcagca gccaagccag ccttcccagc agccttcagt
      421 gcaggcagcc attccccaga cccagcttat gctagctgga ggacagataa ctgggcttac
      481 tttgacgcct gcccagcaac agttactact ccagcaggca caggcacagg cacagctgct
      541 ggctgctgca gtgcagcagc actccgccag ccagcagcac agtgctgctg gagccaccat
      601 ctccgcctct gctgccacgc ccatgacgca gatccccctg tctcagccca tacagatcgc
      661 acaggatctt caacaactgc aacagcttca acagcagaat ctcaacctgc aacagtttgt
      721 gttggtgcat ccaaccacca atttgcagcc agcgcagttt atcatctcac agacgcccca
      781 gggccagcag ggtctcctgc aagcgcaaaa tcttctaacg caactacctc agcaaagcca
      841 agccaacctc ctacagtcgc agccaagcat caccctcacc tcccagccag caaccccaac
      901 a
//
