LOCUS       sequence-86038-e        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 580, Emission: 608.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Shemiakina II, Ermakova GV, Cranfill PJ, Baird MA, Evans RA, 
            Souslova EA, Staroverov DB, Gorokhovatsky AY, Putintseva EV, 
            Gorodnicheva TV, Chepurnykh TV, Strukova L, Lukyanov S, Zaraisky AG,
            Davidson MW, Chudakov DM, Shcherbo D
  TITLE     A monomeric red fluorescent protein with low cytotoxicity.
  JOURNAL   Nat Commun. 2012;3:1204. doi: 10.1038/ncomms2208.
  PUBMED    23149748
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1204. doi"; pages: " 
            10.1038/ncomms2208"
COMMENT     Sequence Label: FusionRed-C1
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        44..94
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     46..66
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      133..142
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             139..834
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
     misc_feature    847..912
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 caaatcaacg gacttccaaa atgtcgtaac aactccgccc catgacgcaa atgggcggta
       61 ggcgtgtacg gtggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      121 gcgctaccgg tcgccaccat ggtgagcgag ctgattaagg agaacatgcc catgaagctg
      181 tacatggagg gcaccgtgaa caaccaccac ttcaagtgca catccgaggg cgaaggcaag
      241 ccctacgagg gcacccagac catgagaatc aaggtcgtcg agggcggccc tctccccttc
      301 gccttcgaca tcctggctac cagcttcatg tacggcagca gaaccttcat caagcaccct
      361 ccgggcatcc ccgacttctt taagcagtcc ttccctgagg gcttcacatg ggagagagtc
      421 accacatacg aagacggggg cgtgctgacc gctacccagg acaccagcct ccaggacggc
      481 tgcctcatct acaacgtcaa ggttagaggg gtgaacttcc cagccaacgg ccctgtgatg
      541 cagaagaaaa cactcggctg ggaggcctcc accgagacga tgtaccccgc tgacggcggc
      601 ctggaaggcg catgtgacat ggccctgaag ctcgtgggcg ggggccacct gatctgcaac
      661 cttgagacca catacagatc caagaaaccc gctacgaacc tcaagatgcc cggcgtctac
      721 aacgtggacc acagactgga aagaatcaag gaggccgacg atgagaccta cgtcgagcag
      781 cacgaggtgg ctgtggccag atactctact ggtggcgctg gtgatggagg taaaggtgga
      841 ggaggttccg gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga cggtaccgcg
      901 ggcccgggat ccaccggatc tagataactg atcataatca gccataccac atttgtagag
      961 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaatgaatg
     1021 caattgttgt tgttaacttg ttattgcagc ttatatggtt acaat
//
