LOCUS       sequence-86039-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 580, Emission: 608.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Shemiakina II, Ermakova GV, Cranfill PJ, Baird MA, Evans RA, 
            Souslova EA, Staroverov DB, Gorokhovatsky AY, Putintseva EV, 
            Gorodnicheva TV, Chepurnykh TV, Strukova L, Lukyanov S, Zaraisky AG,
            Davidson MW, Chudakov DM, Shcherbo D
  TITLE     A monomeric red fluorescent protein with low cytotoxicity.
  JOURNAL   Nat Commun. 2012;3:1204. doi: 10.1038/ncomms2208.
  PUBMED    23149748
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1204. doi"; pages: " 
            10.1038/ncomms2208"
COMMENT     Sequence Label: FusionRed-N1
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        42..92
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     44..64
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    115..195
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      197..206
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             203..901
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
ORIGIN
        1 caaatcaacg gacttccaaa atgtcgtaac actccgccca tgacgcaaat gggcggtagg
       61 cgtgtacggt ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc
      121 gctaccggac tcagatctcg agctcaagct tcgaattctg cagtcgacgg taccgcgggc
      181 ccgggatcca ccggtcgcca ccatggtgag cgagctgatt aaggagaaca tgcccatgaa
      241 gctgtacatg gagggcaccg tgaacaacca ccacttcaag tgcacatccg agggcgaagg
      301 caagccctac gagggcaccc agaccatgag aatcaaggtc gtcgagggcg gccctctccc
      361 cttcgccttc gacatcctgg ctaccagctt catgtacggc agcagaacct tcatcaagca
      421 ccctccgggc atccccgact tctttaagca gtccttccct gagggcttca catgggagag
      481 agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca gcctccagga
      541 cggctgcctc atctacaacg tcaaggttag aggggtgaac ttcccagcca acggccctgt
      601 gatgcagaag aaaacactcg gctgggaggc ctccaccgag acgatgtacc ccgctgacgg
      661 cggcctggaa ggcgcatgtg acatggccct gaagctcgtg ggcgggggcc acctgatctg
      721 caaccttgag accacataca gatccaagaa acccgctacg aacctcaaga tgcccggcgt
      781 ctacaacgtg gaccacagac tggaaagaat caaggaggcc gacgatgaga cctacgtcga
      841 gcagcacgag gtggctgtgg ccagatactc tactggtggc gctggtgatg gaggtaaatg
      901 agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
      961 taaaaaacct ccacacctcc ccctgaacct gaac
//
