LOCUS       sequence-86040-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Aedes aegypti polyubiquitin promoter  followed by MCS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  TITLE     Adelman Lab Vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSLfa-PUb-MCS
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    72..93
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(108..113,114..131,132..138)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    146..162
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     151..173
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     170..186
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     170..186
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(234..253)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    263..344
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     288..307
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
       61 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
      121 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
      181 tatgacatga ttacgaattg gcgcgcctag gccggccgaa ttcaagatac attgatgagt
      241 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
      301 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
      361 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
      421 tctacaaatg tggtatggct gattatgatc tagagtcgcg gccgcgtcga cagatctgca
      481 ggatccatat gccatggttg aaatctctgt tgagcagaaa aagaaacgag gaaacgcttg
      541 agtaattggt tgtgaaatgc aaactctcat ttgatattga ttcattgcct ttggcttcga
      601 gcacgacacg acaggtttta aacttgtttt gctttgtctg cgtttgcagt cgcaggccaa
      661 gtgaaaaata tacacttgaa ggtgatgacg tcacaacaac gccctacttt taagtgaaaa
      721 attaacttgt tttcgacttt gaactacgta gttttgaaat tgcgtatctt caagttttca
      781 cgatttcctc aaggtttttc tcgatatgtg ttaatattac cttaatgggt aattaccatc
      841 aaaatattta tttt
//
