LOCUS       sequence-86044-e         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mos1 transformation vector containing MCS, loxP, attP, 2 x 5HE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Anderson MA, Gross TL, Myles KM, Adelman ZN
  TITLE     Validation of novel promoter sequences derived from two endogenous 
            ubiquitin genes in transgenic Aedes aegypti.
  JOURNAL   Insect Mol Biol. 2010 Aug;19(4):441-9. doi: 
            10.1111/j.1365-2583.2010.01005.x. Epub 2010 Apr 26.
  PUBMED    20456509
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1365-2583.2010.01005.x"; journalName: "Insect Mol Biol"; 
            date: "2010-08"; volume: "19"; issue: "4"; pages: "441-9"
COMMENT     Sequence Label: pMos-3xP3DsRed-attP
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    158..279
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(195..214)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     249..268
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(298..331)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(613..646)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cgagcgcacc gagggccgcc accacctgtt cctgtagcgg ccgcgactct agatcataat
       61 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
      121 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
      181 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
      241 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa agcttatcga tacgcgtata
      301 acttcgtata gcatacatta tacgaagtta tatcgtagca gctcacggtt agggataaca
      361 gggtaattat ttgaggaggt ttctctgtaa ataatgactc tcttaaggta gcctagaaca
      421 aaacgtcgtg agacagtttg gtttaattaa cgcgtacggc gcgccactag tgatatctac
      481 tcgagtgaat tcttatttga ggaggtttct ctgtaaataa tgatcgtagc agctcacggt
      541 tacaaaacgt cgtgagacag tttggtaggg ataacagggt aatgactctc ttaaggtagc
      601 ctagaaagat ctataacttc gtatagcata cattatacga agttatgtag tgccccaact
      661 ggggtaacct ttgagttctc tcagttgggg gcgtagccta ggccggccga tctcgtcgac
      721 gtcaaaatca ccacttttga agcgttgaaa ccaccgttca cacgttttca cagttggtac
      781 ttgttcgcca aaggcttcaa caagcattcg gtgcgattcc gcagctgttt tcttcaaatg
      841 aaa
//
