LOCUS       sequence-86048-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of rice codon-optimized Cas9 in plant cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Shan Q, Wang Y, Li J, Zhang Y, Chen K, Liang Z, Zhang K, Liu J, Xi 
            JJ, Qiu JL, Gao C
  TITLE     Targeted genome modification of crop plants using a CRISPR-Cas 
            system.
  JOURNAL   Nat Biotechnol. 2013 Aug;31(8):686-8. doi: 10.1038/nbt.2650.
  PUBMED    23929338
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2013-08"; volume: "31"; issue:
            "8"; pages: "686-8"
COMMENT     Sequence Label: pJIT163-2NLSCas9
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             63..83
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tttcatttgg agaggacagc ccaagcttcc accatggcgt gcaggtcgac ggatccatgg
       61 cccctaagaa gaagagaaag gtcggtattc acggcgttcc tgcggcgatg gacaagaagt
      121 atagtattgg tctggacatt gggacgaatt ccgttggctg ggccgtgatc accgatgagt
      181 acaaggtccc ttccaagaag tttaaggttc tggggaacac cgatcggcac agcatcaaga
      241 agaatctcat tggagccctc ctgttcgact caggcgagac cgccgaagca acaaggctca
      301 agagaaccgc aaggagacgg tatacaagaa ggaagaatag gatctgctac ctgcaggaga
      361 ttttcagcaa cgaaatggcg aaggtggacg attcgttctt tcatagattg gaggagagtt
      421 tcctcgtcga ggaagataag aagcacgaga ggcatcctat ctttggcaac attgtcgacg
      481 aggttgccta tcacgaaaag taccccacaa tctatcatct gcggaagaag cttgtggact
      541 cgactgataa ggcggacctt agattgatct acctcgctct ggcacacatg attaagttca
      601 ggggccattt tctgatcgag ggggatctta acccggacaa tagcgatgtg gacaagttgt
      661 tcatccagct cgtccaaacc tacaatcagc tctttgagga aaacccaatt aatgcttcag
      721 gcgtcgacgc caaggcgatc ctgtctgcac gcctttcaaa gtctcgccgg cttgagaact
      781 tgatcgctca actcccgggc gaaaagaaga acggcttgtt cgggaa
//
