LOCUS       sequence-86049-e         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of rice codon-optimized Cas9 in plant cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Shan Q, Wang Y, Li J, Zhang Y, Chen K, Liang Z, Zhang K, Liu J, Xi 
            JJ, Qiu JL, Gao C
  TITLE     Targeted genome modification of crop plants using a CRISPR-Cas 
            system.
  JOURNAL   Nat Biotechnol. 2013 Aug;31(8):686-8. doi: 10.1038/nbt.2650.
  PUBMED    23929338
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2013-08"; volume: "31"; issue:
            "8"; pages: "686-8"
COMMENT     Sequence Label: pJIT163-2NLSCas9
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    505..681
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(739..786)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ccggtgtgag ggaactagtt ttgatcttga aagatctttt atctttagag ttaagaactc
       61 tttcgtattt tggtgaggtt ttatcctctt gagttttggt catagaccta ttcatggctc
      121 tgataccaat ttttaagcgg gggcttatgc ggattatttc ttaaattgat aaggggttat
      181 tagggggtat agggtataaa tacaagcatt cccttagcgt atagtataag tatagtagcg
      241 tacctctatc aaatttccat cttcttacct tgcacagggc ctgcaacctt atccttcctt
      301 gtcttcctcc ttccttccgt ccacttcatc atatttaaac caaacctacg ggggagtcaa
      361 cgtaaccaac cctgccttag catcttttcc ctaacggcct cctgcctaag cggtacttct
      421 agcttcgaac ggcgtctggg ctccaggttt agtcgtctcg tgtctggttt atattcacga
      481 caaagatcta tagggacttt aggagatctg gattttagta ctggattttg gttttaggaa
      541 ttagaaattt tattgataga agtattttac aaatacaaat acatactaag ggtttcttat
      601 atgctcaaca catgagcgaa accctataag aaccctaatt tcccttatcg ggaaactact
      661 cacacattat ttatggagaa aatagagaga gatagatttg tagagagaga ctggtgattt
      721 cagcgtaccg aattgctact tcttcttctt cgcttgtcct gccttcttgg ttgccgctgg
      781 tctcttatcc cccccgagct gactcaagtc gatccgcgtc tcgtagaggc cagtaatcga
      841 ttggtggata agggttgcat ccagcacctc cttcgtagaa gtgtatctct tccgatcaat
      901 cgtagtgtca aaatacttga atgcagcggg agccccaagg tttgtcaggg tgaagagatg
      961 aa
//
