LOCUS       sequence-86050-e        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transcription of guide RNA (gRNA) in rice cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Shan Q, Wang Y, Li J, Zhang Y, Chen K, Liang Z, Zhang K, Liu J, Xi 
            JJ, Qiu JL, Gao C
  TITLE     Targeted genome modification of crop plants using a CRISPR-Cas 
            system.
  JOURNAL   Nat Biotechnol. 2013 Aug;31(8):686-8. doi: 10.1038/nbt.2650.
  PUBMED    23929338
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2013-08"; volume: "31"; issue:
            "8"; pages: "686-8"
COMMENT     Sequence Label: pU3-gRNA
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        14..394
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
     misc_RNA        424..499
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(559..575)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(559..575)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(572..594)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    583..599
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(607..613,614..631,632..637))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    652..673
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(790..807)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 caggtcgacg attaaggaat ctttaaacat acgaacagat cacttaaagt tcttctgaag
       61 caacttaaag ttatcaggca tgcatggatc ttggaggaat cagatgtgca gtcagggacc
      121 atagcacaag acaggcgtct tctactggtg ctaccagcaa atgctggaag ccgggaacac
      181 tgggtacgtc ggaaaccacg tgatgtgaag aagtaagata aactgtagga gaaaagcatt
      241 tcgtagtggg ccatgaagcc tttcaggaca tgtattgcag tatgggccgg cccattacgc
      301 aattggacga caacaaagac tagtattagt accacctcgg ctatccacat agatcaaagc
      361 tgatttaaaa gagttgtgca gatgatccgt ggcagctcgc aggtgaacac aacacctgca
      421 cacgttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
      481 agtggcaccg agtcggtgct ttttttccac ataatctcta gaggatcccc gggtaccgag
      541 ctcgaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
      601 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
      661 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
      721 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
      781 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
      841 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
      901 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
      961 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcaga
//
