LOCUS       sequence-86058-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of human 6His-tagged SUMO1, SUMO-E1 and 
            SUMO-E2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Weber AR, Schuermann D, Schar P
  TITLE     Versatile Recombinant SUMOylation System for the Production of 
            SUMO-Modified Protein.
  JOURNAL   PLoS One. 2014 Jul 9;9(7):e102157. doi: 
            10.1371/journal.pone.0102157. eCollection 2014.
  PUBMED    25007328
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-9"; volume: "9(7):e102157. doi"; pages: " 
            10.1371/journal.pone.0102157. eCollection 2014"
COMMENT     Sequence Label: pSUMO1
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        116..134
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    135..159
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             215..232
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        693..711
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    712..736
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ccctgaattg actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt
       61 cgatggtgtc cgggatctcg acgctctccc ttatgcgact cctgcattag gaaattaata
      121 cgactcacta taggggaatt gtgagcggat aacaattccc ctgtagaaat aattttgttt
      181 aactttaata aggagatata ccatgggcag cagccatcac catcatcacc acagccagga
      241 tctggttccg cgtatgtctg accaggaggc caaaccttca actgaggact tgggggataa
      301 gaaggaaggt gaatatatta aactcaaagt cattggacag gatagcagtg agattcactt
      361 caaagtgaaa atgacaacac atctcaagaa actcaaagaa tcatactgtc aaagacaggg
      421 tgttccaatg aattcactca ggtttctctt tgagggtcag agaattgctg ataatcatac
      481 tccaaaagaa ctgggaatgg aggaagaaga tgtgattgaa gtttatcagg aacaaacggg
      541 gggttaggga tccccgggaa ttcatcgtga ctgactgacg ctgcaggtcg acaagcttgc
      601 ggccgcataa tgcttaaccc cttggggcct ctaaacgggt cttgaggggt tttttgcatg
      661 catcgtattg tacacggccg cataatcgaa attaatacga ctcactatag gggaattgtg
      721 agcggataac aattccccat cttagtatat tagttaagta taagaaggag atatacatat
      781 gtcggggatc gccctcagca gactcgccca ggagaggaaa gcatggagga aagaccaccc
      841 atttggtttc gtggctgtcc caacaa
//
