LOCUS       sequence-86060-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of human 6His-tagged SUMO3, SUMO-E1 and 
            SUMO-E2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Weber AR, Schuermann D, Schar P
  TITLE     Versatile Recombinant SUMOylation System for the Production of 
            SUMO-Modified Protein.
  JOURNAL   PLoS One. 2014 Jul 9;9(7):e102157. doi: 
            10.1371/journal.pone.0102157. eCollection 2014.
  PUBMED    25007328
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-9"; volume: "9(7):e102157. doi"; pages: " 
            10.1371/journal.pone.0102157. eCollection 2014"
COMMENT     Sequence Label: pSUMO3
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        111..129
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    130..154
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             210..227
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             249..524
                     /codon_start=1
                     /product="human small ubiquitin-related modifier 3"
                     /label=SUMO3
                     /translation="MSEEKPKEGVKTENDHINLKVAGQDGSVVQFKIKRHTPLSKLMKA
                     YCERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGG"
     promoter        670..688
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    689..713
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg
       61 gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaaat taatacgact
      121 cactataggg gaattgtgag cggataacaa ttcccctgta gaaataattt tgtttaactt
      181 taataaggag atataccatg ggcagcagcc atcaccatca tcaccacagc caggatctgg
      241 ttccgcgtat gtccgaggag aagcccaagg agggtgtgaa gacagagaat gaccacatca
      301 acctgaaggt ggcagggcag gacggctccg tggtgcagtt caagatcaag aggcacacgc
      361 cgctgagcaa gctgatgaag gcctactgcg agaggcaggg cttgtcaatg aggcagatca
      421 gattcaggtt cgacgggcag ccaatcaatg aaactgacac tccagcacag ctggagatgg
      481 aggacgagga caccatcgac gtgttccagc agcagacggg aggttagctc gagcggccgc
      541 atcgtgactg actgacgctg caggtcgaca agcttgcggc cgcataatgc ttaacccctt
      601 ggggcctcta aacgggtctt gaggggtttt ttgcatgcat cgtattgtac acggccgcat
      661 aatcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tccccatctt
      721 agtatattag ttaagtataa gaaggagata tacatatgtc ggggatcgcc ctcagcagac
      781 tcgcccagga gaggaaagca tggaggaaag accacccatt tggtttcgtg gctgtcccaa
      841 caaaa
//
