LOCUS       sequence-86062-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of human 6His-tagged SUMO2, SUMO-E1 and 
            SUMO-E2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Weber AR, Schuermann D, Schar P
  TITLE     Versatile Recombinant SUMOylation System for the Production of 
            SUMO-Modified Protein.
  JOURNAL   PLoS One. 2014 Jul 9;9(7):e102157. doi: 
            10.1371/journal.pone.0102157. eCollection 2014.
  PUBMED    25007328
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-9"; volume: "9(7):e102157. doi"; pages: " 
            10.1371/journal.pone.0102157. eCollection 2014"
COMMENT     Sequence Label: pSUMO2
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        119..137
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    138..162
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             218..235
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             257..535
                     /codon_start=1
                     /product="human small ubiquitin-related modifier 2"
                     /label=SUMO2
                     /translation="MADEKPKEGVKTENNDHINLKVAGQDGSVVQFKIKRHTPLSKLMK
                     AYCERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGG"
     promoter        674..692
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    693..717
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcgcc
       61 attcgatggt gtccgggatc tcgacgctct cccttatgcg actcctgcat taggaaatta
      121 atacgactca ctatagggga attgtgagcg gataacaatt cccctgtaga aataattttg
      181 tttaacttta ataaggagat ataccatggg cagcagccat caccatcatc accacagcca
      241 ggatctggtt ccgcgtatgg ccgacgaaaa gcccaaggaa ggagtcaaga ctgagaacaa
      301 cgatcatatt aatttgaagg tggcggggca ggatggttct gtggtgcagt ttaagattaa
      361 gaggcataca ccacttagta aactaatgaa agcctattgt gaacgacagg gattgtcaat
      421 gaggcagatc agattccgat ttgacgggca accaatcaat gaaacagaca cacctgcaca
      481 gttggaaatg gaggatgaag atacaattga tgtgttccaa cagcagacgg gaggttagga
      541 attcatcgtg actgactgac gctgcaggtc gacaagcttg cggccgcata atgcttaacc
      601 ccttggggcc tctaaacggg tcttgagggg ttttttgcat gcatcgtatt gtacacggcc
      661 gcataatcga aattaatacg actcactata ggggaattgt gagcggataa caattcccca
      721 tcttagtata ttagttaagt ataagaagga gatatacata tgtcggggat cgccctcagc
      781 agactcgccc aggagaggaa agcatggagg aaagaccacc catttggttt cgtggctgtc
      841 ccaacaaaaa atcccgatgg cacgatgaac ctcatgaa
//
