LOCUS       sequence-86077-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Hong TT, Smyth JW, Gao D, Chu KY, Vogan JM, Fong TS, Jensen BC, 
            Colecraft HM, Shaw RM
  TITLE     BIN1 localizes the L-type calcium channel to cardiac T-tubules.
  JOURNAL   PLoS Biol. 2010 Feb . 8(2):e1000312.
  PUBMED    20169111
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Biol. 
            2010 Feb . 8(2):e1000312."
COMMENT     Sequence Label: pDest-mCherry-N1
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(106..112,113..130,131..136))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(161..285)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(319..522)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(348..368)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        523..826
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
       61 ccttagctcc tgaaaatctc gacggatcct aactcaaaat ccacacatta tacgagccgg
      121 aagcataaag tgtaaagcct ggggtgccta atgcggccgc catagtgact ggatatgttg
      181 tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt
      241 tatatcattt tacgtttctc gttcagcttt tttgtacaaa cttgtttgat gctagcggat
      301 ctgacggttc actaaaccag ctctgcttat atagacctcc caccgtacac gcctaccgcc
      361 catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt gattttggtg
      421 ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa
      481 ccgctatcca cgcccattga tgtactgcca aaaccgcatc accatggtaa tagcgatgac
      541 taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg
      601 ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac
      661 ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa
      721 agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc
      781 gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata
      841 tgggctatga actaatgacc ccgtaattga ttactattaa taactaatgc atggcggtaa
      901 tacggt
//
