LOCUS       sequence-86081-e        1042 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Hong TT, Smyth JW, Gao D, Chu KY, Vogan JM, Fong TS, Jensen BC, 
            Colecraft HM, Shaw RM
  TITLE     BIN1 localizes the L-type calcium channel to cardiac T-tubules.
  JOURNAL   PLoS Biol. 2010 Feb . 8(2):e1000312.
  PUBMED    20169111
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Biol. 
            2010 Feb . 8(2):e1000312."
COMMENT     Sequence Label: pDest-mCherry-N1
FEATURES             Location/Qualifiers
     source          1..1042
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      123..132
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    146..270
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(311..616)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(413..432)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ggggcggccc tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg agccctccat
       61 gtgcaccttg aagcgcatga actccttgat gatggccatg ttatcctcct cgcccttgct
      121 caccatggtg gcgggatccc atcgaaccac tttgtacaag aaagctgaac gagaaacgta
      181 aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata
      241 ctgtaaaaca caacatatcc agtcactatg gtcgacctgc agactggctg tgtataaggg
      301 agcctgacat ttatattccc cagaacatca ggttaatggc gtttttgatg tcattttcgc
      361 ggtggctgag atcagccact tcttccccga taacggagac cggcacactg gccatatcgg
      421 tggtcatcat gcgccagctt tcatccccga tatgcaccac cgggtaaagt tcacggggga
      481 ctttatctga cagcagacgt gcactggcca gggggatcac catccgtcgc ccgggcgtgt
      541 caataatatc actctgtaca tccacaaaca gacgataacg gctctctctt ttataggtgt
      601 aaaccttaaa ctgcatttca ccagcccctg ttctcgtcag caaaagagcc gttcatttca
      661 ataaaccggg cgacctcagc catcccttcc tgattttccg ctttccagcg ttcggcacgc
      721 agacgacggg cttcattctg catggttgtg cttaccagac cggagatatt gacatcatat
      781 atgccttgag caactgatag ctgtcgctgt caactgtcac tgtaatacgc tgcttcatag
      841 catacctctt tttgacatac ttcgggtata catatcagta tatattctta taccgcaaaa
      901 atcagcgcgc aaatacgcat actgttatct ggcttttagt aagccggatc ctctagatta
      961 cgccccgcct gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg
     1021 aagccatcac aaacggcatg at
//
