LOCUS       sequence-86087-e        1009 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  plasmid contains human HCFC1 full length gene with HA and c-myc 
            tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Wilson AC, LaMarco K, Peterson MG, Herr W
  TITLE     The VP16 accessory protein HCF is a family of polypeptides processed
            from a large precursor protein.
  JOURNAL   Cell. 1993 Jul 16;74(1):115-25.
  PUBMED    8392914
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "1993-07-16"; volume: "74(1)"; pages: "115-25"
COMMENT     Sequence Label: pCGN-HCF-1 fl
FEATURES             Location/Qualifiers
     source          1..1009
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..27
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           96..135
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             165..191
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             171..197
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
       61 catagaagac accgggaccg atccagcctc cgggggatct tggtggcgtg aaactcccgc
      121 acctcttcgg ccagcgcctt gtagaagcgc gtatggcttc tagctatcct tatgacgtgc
      181 ctgactatgc cagcctggga ggaccttcta gatcacgtcg tgcatctggt acgtacggac
      241 gcgtcgcttc ggccgtgtcg cccgccaact tgccagcggt gcttctgcag ccccgctgga
      301 agcgagtggt gggctggtcg ggtccggtgc cacggccccg ccacggccac cgcgccgtgg
      361 ccatcaagga gctcatcgtg gtgtttggcg gcggcaacga gggaatagtg gacgaactgc
      421 acgtgtacaa cacggcaacc aaccagtggt tcatcccagc cgtgaggggg gacattcccc
      481 ctgggtgtgc agcctatggc ttcgtgtgtg acgggactcg cctcctggtg tttggtggga
      541 tggtggagta tgggaaatac agcaatgacc tctacgaact ccaggcgagc cggtgggagt
      601 ggaagagact caaagcaaag acgcccaaaa acgggccccc tccgtgtcct cgactcgggc
      661 acagcttctc ccttgtgggc aacaaatgct acctgtttgg gggtctggcc aatgatagcg
      721 aggacccaaa gaacaacatt ccaaggtacc tgaatgactt atatatcctg gaattacggc
      781 caggctctgg agtggtagcc tgggacattc ccatcactta cggggtccta ccaccacccc
      841 gggagtcaca tactgccgtg gtctacaccg aaaaagacaa taagaagtcc aagctggtga
      901 tctacggcgg gatgagtggc tgcaggctgg gggacctgtg gaccctagat attgacaccc
      961 tgacgtggaa taagcccagt ctcagcgggg tggcgcctct tcctcgcag
//
