LOCUS       sequence-86090-e        1054 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian cell expression vector with HA-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Tanaka M, Herr W
  TITLE     Differential transcriptional activation by Oct-1 and Oct-2: 
            interdependent activation domains induce Oct-2 phosphorylation.
  JOURNAL   Cell. 1990 Feb 9;60(3):375-86.
  PUBMED    2302733
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "1990-02-9"; volume: "60(3)"; pages: "375-86"
COMMENT     Sequence Label: pCGN
FEATURES             Location/Qualifiers
     source          1..1054
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..30
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           99..138
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             168..194
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             174..200
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     intron          254..826
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(958..977)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
ORIGIN
        1 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
       61 ctccatagaa gacaccggga ccgatccagc ctccggggga tcttggtggc gtgaaactcc
      121 cgcacctctt cggccagcgc cttgtagaag cgcgtatggc ttctagctat ccttatgacg
      181 tgcctgacta tgccagcctg ggaggacctt ctagaattcg agctcggtac ccggggatcc
      241 tgagaacttc agggtgagtt tggggaccct tgattgttct ttctttttcg ctattgtaaa
      301 attcatgtta tatggagggg gcaaagtttt cagggtgttg tttagaatgg gaagatgtcc
      361 cttgtatcac catggaccct catgataatt ttgtttcttt cactttctac tctgttgaca
      421 accattgtct cctcttattt tcttttcatt ttctgtaact ttttcgttaa actttagctt
      481 gcatttgtaa cgaattttta aattcacttt tgtttatttg tcagattgta agtactttct
      541 ctaatcactt ttttttcaag gcaatcaggg tatattatat tgtacttcag cacagtttta
      601 gagaacaatt gttataatta aatgataagg tagaatattt ctgcatataa attctggctg
      661 gcgtggaaat attcttattg gtagaaacaa ctacaccctg gtcatcatcc tgcctttctc
      721 tttatggtta caatgatata cactgtttga gatgaggata aaatactctg agtccaaacc
      781 gggcccctct gctaaccatg ttcatgcctt cttctctttc ctacagctcc tgggcaacgt
      841 gctggttgtt gtgctgtctc atcattttgg caaagaattc actcctcagg tgcaggctgc
      901 ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct
      961 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg
     1021 ctaataaagg aaatttattt tcattgcaat agtg
//
