LOCUS       sequence-86093-e        5527 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Aedes aegypti NPY-like receptor AAEL012053 (NPYLR3) full-length 
            clone in mammalian expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Liesch J, Bellani LL, Vosshall LB
  TITLE     Functional and genetic characterization of neuropeptide Y-like 
            receptors in Aedes aegypti.
  JOURNAL   PLoS Negl Trop Dis. 2013 Oct 10;7(10):e2486. doi: 
            10.1371/journal.pntd.0002486. eCollection 2013.
  PUBMED    24130914
REFERENCE   2  (bases 1 to 5527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2013-10-10"; volume: "7(10):e2486. doi"; pages: "
            10.1371/journal.pntd.0002486. eCollection 2013"
COMMENT     Sequence Label: pME18S-12053
FEATURES             Location/Qualifiers
     source          1..5527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(13..33)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(18..347,348..363,364..632)
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      198..333
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             364..632
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          662..755
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             complement(2137..2148)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3432..3566
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3469..3488)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3523..3542
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3605..3622)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3776..4364)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3856..3875)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4535..5326,5327..5395))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5158..5177
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5396..5500)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aagcttggct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
       61 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
      181 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
      241 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
      301 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcctccgat
      361 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc
      421 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc
      481 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta
      541 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt
      601 gtttcgtttt ctgttctgcg ccgttacaga tccaagctct gaaaaaccag aaagttaact
      661 ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg caaatcaaag
      721 aactgctcct cagtggatgt tgcctttact tctaggcctg tacggaagtg ttacttctgc
      781 tctaaaagct gcggaattcc tcgagatgaa gtccaaggag accgcgtcgg atgcgattcc
      841 gatgatcgct cagatgattc tcgggagttt cgaggcgcac aatggctctg caaatgtgga
      901 ctacagtagt ccggtgctga aaccggtggt ggcacgaatc tatcccgtgt tcatactgct
      961 gtacttcatt ccggccgctt tgggcatagc cagtaatgtg ctggtggtga tgtacatcag
     1021 tcggtacaaa ctgtaccggg aggcgacgca ggcctttatg gtcaatctgg ccgtgtgtca
     1081 tattgtgcag tgcgcgttcg tgcttccgat cacgctgatg gtgatgctga tccagaactg
     1141 gatcttcggg cagttcctgt gcttctttct gccgctgctg caggacatcc cgctgcacgt
     1201 cgccgtgatt tcccatctgt tgattgcgtg ggaccggaag cgctggctga cggatccact
     1261 caaggcccgg ctgccggcgt tcgtgtgcag ctgtgcctcc tggctggccg ggatggtgat
     1321 tgcgcttccg tatccgatct acaccaccta tctggatctg gggaaataca tgccggagtt
     1381 cgaaggtgtg gggctttgtg ccctaaatct gatcgacgat atgcaggagt acatgcgggg
     1441 attattcctc attatgtact gtggccccat gacgctgctc gggtatctct acataaggac
     1501 gtctcgggaa ctgcggccac ccgatgggcc cctctcgatt atgatgttcg aagcgagggc
     1561 tgaggccagg agcaagcaac tacgggccag tcgacacgga acgagaagtg gttcaactgg
     1621 cggcagcgct ggagcatccg gagcggattt gagtgccggg tcacggactt acgatttgta
     1681 cgatgccgag gtggacgtga gccgggaaaa gcgaacgcag cgacacctgg gagcgatggc
     1741 ctcgacgcag attttgtgcg tctgtccgct gatgattttg aggttggccc gcatggcact
     1801 ggaggaaacg tacgaaagtg aggcccattt tgacataacc tatctgatgt tcgtgtggat
     1861 ggcattcctg ccgacggtca ttgtgccctg gatctacgcc aactgggtgc tgtcgaggcc
     1921 tgctaaggag cgccttcggg gctacctccg gttatcctcc cgccgcaaca ccggaaccaa
     1981 gtccgaacaa tccctggacg acaacagttc cccacccggc gtgaaaaccc ccctctccac
     2041 gcagatgtcc acggtaagcc atccaacgga gtcggctcca cccaccctgg aagccccccc
     2101 atctgtccac gctccctcgc atccacgcaa caagtcccta ccctcgattg accgttgcaa
     2161 gtcacccgcc ggggtaagca ccaagaccca gcaatccaca ctggagagca ccatcgctga
     2221 gagtgccctc tccgctaccg gagttgccaa aagcacatct accacacgac cccgcaggca
     2281 acttccgccg ccgctgcgga tcgagcacag caacttcgac aaaactcctt ccgcgctgcc
     2341 ccgcaactca tcgggatcta cagttcaacg ctcctcgctg aagtccacca gctccggaag
     2401 tggcggtcga acttcagagc gaacccttcc tccgaatccc aaacccaaaa ccgtcgaaaa
     2461 acccccggag gaagaagacg actactgttc atcgatagcg ggccacttct ccaacataac
     2521 ctccagcacg gactgcaaca tcaacgagcg ggaagtcgtc gccctggagc tgtacgaacc
     2581 gcttcacgcc gtcccacgac aaaacgtcct ctacaaatcg gcgtctccgt actaccaagg
     2641 cggcaacgaa tccaacgact ccgattcgga tatctaccaa aggcgatctg gtcgctacgg
     2701 ctctctgtcc aagccggact ccggcatcct caagggaact ccatcgatca tctccgtcac
     2761 cgagagcgag tactcctccc ggaaaggttc cctcagtact ctctcgcggg acatggagat
     2821 aatcgaccga ctcgaacgcg aacgcagcat ggacatccag gagatgatcc agcgggagaa
     2881 ctacgagtac tacctatcgc ggcagaactc caagttcaag ttctccacca acttcgacga
     2941 ttacttcgaa gaggagccgc cgacggtccc tgcgcctacc ggagttggac caatctcgca
     3001 gaaaccgcgt cccgtttcgc cgcacatggt ccgtaacgcg gcgaatcgtc ggagcaactc
     3061 tagttacgcc tcggccaagc gggacagcgg ctacgagact tcgttgccac cgtcgcaacc
     3121 ggttgtcggg aagaacggtg cgccgcccct tctctacagt ggaccacgga ggtcgctgga
     3181 gctggagagg caccaacacc atcagcagca gcagcggttc aataggacgc acagtgcgca
     3241 ctcggcgcga ggatcgacca agtctaggga tcgggttagc tttcgggata atttgtaaga
     3301 ggaccaacca acagaacgag attgcggtgc caaagattac gggcgaggcg gccgctagac
     3361 tagtctagag aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     3421 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     3481 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     3541 tcaatgtatc ttatcatgtc tggatccccg ggtaccgagc tcgaattaat tcctcttccg
     3601 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     3661 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     3721 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     3781 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     3841 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     3901 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     3961 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     4021 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     4081 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     4141 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     4201 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     4261 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     4321 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     4381 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     4441 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     4501 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     4561 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     4621 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     4681 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     4741 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     4801 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     4861 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     4921 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     4981 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     5041 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     5101 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     5161 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     5221 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     5281 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     5341 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     5401 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     5461 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     5521 cacctgc
//
