LOCUS       sequence-8612-ex        2349 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  modified pENTR vector for N-terminal STREP tag fusion with your gene
            of interest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Dubin MJ, Bowler C, Benvenuto G
  TITLE     A modified Gateway cloning strategy for overexpressing tagged 
            proteins in plants.
  JOURNAL   Plant Methods. 2008 Jan 22. 4(1):3.
  PUBMED    18211686
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods. 2008 Jan 22. 4(1):3."
COMMENT     Sequence Label: pE1n
FEATURES             Location/Qualifiers
     source          1..2349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             484..507
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    complement(578..677)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(789..808)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             800..1609
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(867..886)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1699..2287
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2188..2207
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggcttta aaggaaccaa ttcagtcgac
      481 atgtggagcc atccgcagtt cgaaaaaggc ggcagcggcg gatccggtac agatttcgga
      541 catgcggccg cataagtagc tgactcgaga tatctagacc cagctttctt gtacaaagtt
      601 ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa
      661 aataaaatca ttatttgcca tccagctgca gctctggccc gtgtctcaaa atctctgatg
      721 ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa
      781 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa
      841 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
      901 aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca
      961 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac
     1021 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt
     1081 actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc
     1141 aggtgaaaat attgttgatg cgctggcagt gtccctgcgc cggttgcatt cgattcctgt
     1201 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat
     1261 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga
     1321 acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca
     1381 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga
     1441 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct
     1501 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc
     1561 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt
     1621 taattggttg taacattatt cagattgggc cccgttccac tgagcgtcag acccggtaga
     1681 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     1741 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     1801 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     1861 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     1921 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     1981 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     2041 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     2101 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     2161 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     2221 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     2281 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     2341 tcacatgtt
//
