LOCUS       sequence-86128-e       12624 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus containing mouse Hey2 coding region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12624)
  AUTHORS   Hartman ME, Librande JR, Medvedev IO, Ahmad RN, Moussavi-Harami F, 
            Gupta PP, Chien WM, Chin MT
  TITLE     An optimized and simplified system of mouse embryonic stem cell 
            cardiac differentiation for the assessment of differentiation 
            modifiers.
  JOURNAL   PLoS One. 2014 Mar 25;9(3):e93033. doi: 
            10.1371/journal.pone.0093033. eCollection 2014.
  PUBMED    24667642
REFERENCE   2  (bases 1 to 12624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-03-25"; volume: "9(3):e93033. doi"; pages: " 
            10.1371/journal.pone.0093033. eCollection 2014"
COMMENT     Sequence Label: iDuet101a-mCHF1
FEATURES             Location/Qualifiers
     source          1..12624
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2664..2682,2683..2705,2706..2724,2725..2747,2748..2766,
                     2767..2789,2790..2808,2809..2831,2832..2850,2851..2873,
                     2874..2892,2893..2915,2916..2934)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        3190..4368
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3421..4359
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4316..4336
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     4385..4404
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4385..4403
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      5727..5736
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5733..5735,5736..5738,5739..6446)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     CDS             join(5733..5735,5736..5738,5739..6446)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(5778..5799)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6039..6058)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6386..6407
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        6470..6969
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6505..6528)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6902..6921
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     protein_bind    complement(8371..8404)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             8429..8609
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8635..8652)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8641..8865
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8911..9339
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8998..9017)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9208..9229
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9348..9368)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9353..9682
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9533..9668
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9595..9614
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9730..9777
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9796..10170
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10300..10421
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10337..10356)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10391..10410
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10470..10486)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10470..10486)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10483..10505)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10494..10510
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10518..10524,10525..10542,10543..10548))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10563..10584
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10701..10718)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10872..11460)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10952..10971)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11631..12422,12423..12491))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12254..12273
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12492..12596)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaccag tgtgatggat atctgcagaa ttcgcccttg gatccgaatt
     2641 cctgcagccc cgactttcac ttttctctat cactgatagg gagtggtaaa ctcgactttc
     2701 acttttctct atcactgata gggagtggta aactcgactt tcacttttct ctatcactga
     2761 tagggagtgg taaactcgac tttcactttt ctctatcact gatagggagt ggtaaactcg
     2821 actttcactt ttctctatca ctgataggga gtggtaaact cgactttcac ttttctctat
     2881 cactgatagg gagtggtaaa ctcgactttc acttttctct atcactgata gggagtggta
     2941 aactcgaggg ggatccacta gcatgaaggg cgaattccag cacactggta acccgtgtcg
     3001 gctccagatc tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac
     3061 ggcgagccgc gttgacattg attattgact aggcttttgc aaaaagcttt gcaaagatgg
     3121 ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt cttgaaagga
     3181 gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     3241 aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac
     3301 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     3361 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     3421 gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg
     3481 ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga
     3541 agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt
     3601 gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt
     3661 ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt
     3721 tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt
     3781 tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg
     3841 ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct
     3901 ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg
     3961 tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca
     4021 aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg
     4081 gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg
     4141 cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt
     4201 tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac
     4261 ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag
     4321 cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgagg aattagcttg
     4381 gtactaatac gactcactat agggagaccc aagctggcta ggtaagcttg gtaccgagct
     4441 cggatccact agtccagtgt ggtggaattc tgcagatatc cagcacagtg gcggccgctc
     4501 gagtctagag aattccggga gcgcgctgcg ttccgctagg cgacagtagc tgctcctcct
     4561 tcgtcgcgat gaagcgccct tgtgaggaaa cgacctccga aagcgacctg gacgagacca
     4621 tcgacgtggg gagcgagaac aattaccctg ggcacgctac aagttcagtg atgaggtcca
     4681 attcaccgac aactacctct cagattatgg caagaaagaa aaggagaggg atcatagaaa
     4741 aaaggcgtcg ggatcgaata aataacagtt tatctgaatt gagaagacta gtgccaacag
     4801 cttttgaaaa acaaggatct gccaagttag aaaaggctga aatattgcaa atgacagtgg
     4861 atcatttgaa gatgctccag gctacagggg gtaaaggcta ctttgatgcc catgctcttg
     4921 ccacagactt catgagcatt ggattccgag agtgcttgac agaagtggct aggtacctaa
     4981 gctcagtgga aggccttgac ccgtcggacc cactacgcgt gcgccttgtc tctcatctca
     5041 gcacctgtgc ctcccagcgg gaggcagcag tgatgacatc ctccatggcc caccaccatc
     5101 accccttgca ccctcaccac tgggcagctg ctttccacca tctccccaca gccctgctcc
     5161 agcccaatgg actccacaca tcagagtcaa ccccatgtcg cctatccaca tcttcagaag
     5221 tgccttctgc tcatggctct gctctcctca cagcaacgtt tgcccatgca gattctgctc
     5281 ttcggatgcc atcagggggc accgttgcac cctgcgtgcc acctctctcc acctctcttc
     5341 tgtctctttc ggccactgtg catgccgcag ctgcagcagc cactgcagct gcacacagct
     5401 tccctctgtc cttcgcaggg gcttttccca tgctcccgtc caatgcagcg gcagcagccg
     5461 ctgttgctgc tgcaacagca atcagcccac ccttgtcggt atccgcagcc tccagtcctc
     5521 agcagacaag cactgggaca aacaataaac cttaccaacc ctgggggaca gaagttggag
     5581 ccttttaact tattcttgaa tctctccgga attccgccga tactgacggg ctccaggagt
     5641 cgtcgccacc aagggcgaat tctgcagata tccatcacac tggcggccgc tcgagcatgc
     5701 atctagagga tccccgggta ccggtcgcca ccatggtgag caagggcgag gagctgttca
     5761 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     5821 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     5881 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     5941 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     6001 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     6061 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     6121 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     6181 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     6241 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     6301 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     6361 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     6421 tcactctcgg catggacgag ctgtacactc gaggttaacg aattctaccg ggtaggggag
     6481 gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc
     6541 tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct
     6601 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc
     6661 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt
     6721 ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc
     6781 caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg
     6841 ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg
     6901 gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg
     6961 ggcctttcga cctgcatccc gccaccatga aaaagcctga actcaccgcg acgtctgtcg
     7021 agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg
     7081 aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata
     7141 gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc
     7201 tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct
     7261 cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc
     7321 tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg
     7381 ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat
     7441 gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg
     7501 cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc
     7561 ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa
     7621 cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca
     7681 tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga
     7741 ggcatccgga gcttgcagga tcgccgcggc tccggggcgt atatgctccg cattggtctt
     7801 gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt
     7861 cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc
     7921 agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga
     7981 cgccccagca ctcgtccgag ggcaaaggaa tagagtagat gccgaccgaa caagagctga
     8041 tttcgagaac gcctcagcca gcaactcgcg cgagcctagc aaggcaaatg cgagagaacg
     8101 gccttacgct tggtggcaca gttctcgtcc acagttcgct aagctcgctc ggctgggtcg
     8161 cgggagggcc ggtcgcagtg attcaggccc ttctggattg tgttggtccc cagggcacga
     8221 ttgtcatgcc cacgcactcg ggtgatctga ctgatcccgc agattggaga tcgccgcccg
     8281 tgcctgccga ttgggtgcag atccgtcgag ttaacaaaag aaaagggggg actggaaggg
     8341 ctaattcact cccaacgaag acaagatatc ataacttcgt atagcataca ttatacgaag
     8401 ttatcggcta gctggtccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg
     8461 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     8521 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     8581 cttttagtca gtgtggaaaa tctctagcag ggcccgttta aacccgctga tcagcctcga
     8641 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     8701 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     8761 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     8821 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     8881 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     8941 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     9001 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     9061 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     9121 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     9181 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     9241 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     9301 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     9361 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     9421 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     9481 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     9541 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     9601 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     9661 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     9721 tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag
     9781 gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac
     9841 gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag
     9901 gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac
     9961 caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac
    10021 gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc
    10081 gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc
    10141 gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc
    10201 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
    10261 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
    10321 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
    10381 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
    10441 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
    10501 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
    10561 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
    10621 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
    10681 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
    10741 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
    10801 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
    10861 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
    10921 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
    10981 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
    11041 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
    11101 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
    11161 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
    11221 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
    11281 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
    11341 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
    11401 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
    11461 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
    11521 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
    11581 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
    11641 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
    11701 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
    11761 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
    11821 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
    11881 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    11941 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    12001 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    12061 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    12121 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    12181 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    12241 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    12301 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    12361 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    12421 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    12481 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    12541 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    12601 tttccccgaa aagtgccacc tgac
//
