LOCUS       sequence-86130-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  destination vector with cis-golgi marker (alpha-man I 49AA) -ECFP 
            for tagged fluorescent protein (C-ter-EYFP) of plant gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Lao J, Oikawa A, Bromley JR, McInerney P, Suttangkakul A, 
            Smith-Moritz AM, Plahar H, Chiu TY, Gonzalez Fernandez-Nino SM, 
            Ebert B, Yang F, Christiansen KM, Hansen SF, Stonebloom S, Adams PD,
            Ronald PC, Hillson NJ, Hadi MZ, Vega-Sanchez ME, Loque D, Scheller 
            HV, Heazlewood1 JL
  TITLE     The Plant Glycosyltransferase Clone Collection for Functional 
            Genomics.
  JOURNAL   Plant J. 2014 Jun 6. doi: 10.1111/tpj.12577.
  PUBMED    24905498
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2014 Jun 6. doi: 10.1111/tpj.12577."
COMMENT     Sequence Label: pBullet-cg-c
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(33..135)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    complement(313..437)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(493..514)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(754..773)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
       61 gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct
      121 cttacgtgcc gatcaacgtc tcattttcgc caaaagttgg cccagggctt cccggtatca
      181 acagggacac caggatttat ttattctgcg aagtgatctt ccgtcacagg tatttattcg
      241 gcgcaaagtg cgtcgggtga tgctgccaac ttagtcgact acaggtcact aataccatct
      301 aagtagttga ttcatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt
      361 ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct
      421 ttcttgtaca aagtggttgc cacggccatg gtgagcaagg gcgaggagct gttcaccggg
      481 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      541 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      601 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgcagtgc
      661 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      721 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      781 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaa
//
