LOCUS       sequence-86139-e        1132 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HA tagged human MEK1 from blasticidin resistance 
            retroviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Brady DC, Crowe MS, Turski ML, Hobbs GA, Yao X, Chaikuad A, Knapp S,
            Xiao K, Campbell SL, Thiele DJ, Counter CM
  TITLE     Copper is required for oncogenic BRAF signalling and tumorigenesis.
  JOURNAL   Nature. 2014 Apr 9. doi: 10.1038/nature13180.
  PUBMED    24717435
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Apr 9. doi: 10.1038/nature13180."
COMMENT     Sequence Label: pWZLblasti-HA-MEK1
FEATURES             Location/Qualifiers
     source          1..1132
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..23
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             68..94
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tcctcccttt atccagccct cactccttct ctaggcgccg gccggatccg atatcgccgc
       61 caccatgtac ccatacgatg ttccagatta cgctcccaag aagaagccga cgcccatcca
      121 gctgaacccg gcccccgacg gctctgcagt taacgggacc agctctgcgg agaccaactt
      181 ggaggccttg cagaagaagc tggaggagct agagcttgat gagcagcagc gaaagcgcct
      241 tgaggccttt cttacccaga agcagaaggt gggagaactg aaggatgacg actttgagaa
      301 gatcagtgag ctgggggctg gcaatggcgg tgtggtgttc aaggtctccc acaagccttc
      361 tggcctggtc atggccagaa agctaattca tctggagatc aaacccgcaa tccggaacca
      421 gatcataagg gagctgcagg ttctgcatga gtgcaactct ccgtacatcg tgggcttcta
      481 tggtgcgttc tacagcgatg gcgagatcag tatctgcatg gagcacatgg atggaggttc
      541 tctggatcaa gtcctgaaga aagctggaag aattcctgaa caaattttag gaaaagttag
      601 cattgctgta ataaaaggcc tgacatatct gagggagaag cacaagatca tgcacagaga
      661 tgtcaagccc tccaacatcc tagtcaactc ccgtggggag atcaagctct gtgactttgg
      721 ggtcagcggg cagctcatcg actccatggc caactccttc gtgggcacaa ggtcctacat
      781 gtcgccagaa agactccagg ggactcatta ctctgtgcag tcagacatct ggagcatggg
      841 actgtctctg gtagagatgg cggttgggag gtatcccatc cctcctccag atgccaagga
      901 gctggagctg atgtttgggt gccaggtgga aggagatgcg gctgagaccc cacccaggcc
      961 aaggaccccc gggaggcccc ttagctcata cggaatggac agccgacctc ccatggcaat
     1021 ttttgagttg ttggattaca tagtcaacga gcctcctcca aaactgccca gtggagtgtt
     1081 cagtctggaa tttcaagatt ttgtgaataa atgcttaata aaaaaccccg ca
//
