LOCUS       sequence-86140-e        1133 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human CTR1 M154A from blasticidin resistance retroviral 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Brady DC, Crowe MS, Turski ML, Hobbs GA, Yao X, Chaikuad A, Knapp S,
            Xiao K, Campbell SL, Thiele DJ, Counter CM
  TITLE     Copper is required for oncogenic BRAF signalling and tumorigenesis.
  JOURNAL   Nature. 2014 Apr 9. doi: 10.1038/nature13180.
  PUBMED    24717435
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Apr 9. doi: 10.1038/nature13180."
COMMENT     Sequence Label: pWZLblasti-CTR1M154A
FEATURES             Location/Qualifiers
     source          1..1133
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..23
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      53..62
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(738..755)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     965..984
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 tcctcccttt atccagccct cactccttct ctaggcgccg gccggatccg ccgccaccat
       61 ggatcattcc caccatatgg ggatgagcta tatggactcc aacagtacca tgcaaccttc
      121 tcaccatcac ccaaccactt cagcctcaca ctcccatggt ggaggagaca gcagcatgat
      181 gatgatgcct atgaccttct actttggctt taagaatgtg gaactactgt tttccggttt
      241 ggtgatcaat acagctggag aaatggctgg agcttttgtg gcagtgtttt tactagcatt
      301 gttctatgaa ggactcaaga tagcccgaga gagcctgctg cgtaagtcac aagtcagcat
      361 tcgctacaat tccatgcctg tcccaggacc aaatggaacc atccttatgg agacacacaa
      421 aactgttggg caacagatgc tgagctttcc tcacctcctg caaacagtgc tgcacatcat
      481 ccaggtggtc ataagctact tcctcatgct catcttcgcg acctacaacg ggtacctctg
      541 cattgcagta gcagcagggg ccggtacagg atacttcctc ttcagctgga agaaggcagt
      601 ggtagtggat atcacagagc attgccattg agtcgaccaa ttccggttat tttccaccat
      661 attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat
      721 tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga
      781 agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca
      841 gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac
      901 acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt
      961 caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca
     1021 ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt
     1081 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aac
//
