LOCUS       sequence-86143-e        1186 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of IFIT1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Katibah GE, Lee HJ, Huizar JP, Vogan JM, Alber T, Collins K
  TITLE     tRNA binding, structure, and localization of the human 
            interferon-induced protein IFIT5.
  JOURNAL   Mol Cell. 2013 Feb 21;49(4):743-50. doi: 
            10.1016/j.molcel.2012.12.015. Epub 2013 Jan 11.
  PUBMED    23317505
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2012.12.015"; journalName: "Mol Cell"; date: 
            "2013-02-21- 21"; volume: "49"; issue: "4"; pages: "743-50"
COMMENT     Sequence Label: pET28a(+) 6xHis-TEV-IFIT1
FEATURES             Location/Qualifiers
     source          1..1186
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     69..88
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        69..87
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    88..112
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             127..149
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             168..185
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             195..212
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             228..248
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc
       61 cgcgaaatta atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga
      121 aataattttg tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc
      181 atcacagcag cggcctggtg ccgcgcggca gccatatggc tagcagagaa aacctgtatt
      241 ttcagagtac aaatggtgat gatcatcagg tcaaggatag tctggagcaa ttgagatgtc
      301 actttacatg ggagttatcc attgatgacg atgaaatgcc tgatttagaa aacagagtct
      361 tggatcagat tgaattccta gacaccaaat acagtgtggg aatacacaac ctactagcct
      421 atgtgaaaca cctgaaaggc cagaatgagg aagccctgaa gagcttaaaa gaagctgaaa
      481 acttaatgca ggaagaacat gacaaccaag caaatgtgag gagtctggtg acctggggca
      541 actttgcctg gatgtattac cacatgggca gactggcaga agcccagact tacctggaca
      601 aggtggagaa catttgcaag aagctttcaa atcccttccg ctatagaatg gagtgtccag
      661 aaatagactg tgaggaagga tgggccttgc tgaagtgtgg aggaaagaat tatgaacggg
      721 ccaaggcctg ctttgaaaag gtgcttgaag tggaccctga aaacccggaa tccagcgctg
      781 ggtatgcgat ctctgcctat cgcctggatg gctttaaatt agccacaaaa aatcacaagc
      841 cattttcttt gcttccccta aggcaggctg tccgcttaaa tccagacaat ggatatatta
      901 aggttctcct tgccctgaag cttcaggatg aaggacagga agctgaagga gaaaagtaca
      961 ttgaagaagc tctagccaac atgtcctcac agacctatgt ctttcgatat gcagccaagt
     1021 tttaccgaag aaaaggctct gtggataaag ctcttgagtt attaaaaaag gccttgcagg
     1081 aaacacccac ttctgtctta ctgcatcacc agatagggct ttgctacaag gcacaaatga
     1141 tccaaatcaa ggaggctaca aaagggcagc ctagagggca gaacag
//
