LOCUS       sequence-86160-e        1134 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of IFIT3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Katibah GE, Lee HJ, Huizar JP, Vogan JM, Alber T, Collins K
  TITLE     tRNA binding, structure, and localization of the human 
            interferon-induced protein IFIT5.
  JOURNAL   Mol Cell. 2013 Feb 21;49(4):743-50. doi: 
            10.1016/j.molcel.2012.12.015. Epub 2013 Jan 11.
  PUBMED    23317505
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2012.12.015"; journalName: "Mol Cell"; date: 
            "2013-02-21- 21"; volume: "49"; issue: "4"; pages: "743-50"
COMMENT     Sequence Label: pET28a(+) 6xHis-TEV-IFIT3
FEATURES             Location/Qualifiers
     source          1..1134
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     69..88
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        69..87
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    88..112
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             127..149
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             168..185
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             195..212
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             228..248
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc
       61 cgcgaaatta atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga
      121 aataattttg tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc
      181 atcacagcag cggcctggtg ccgcgcggca gccatatggc tagcagagaa aacctgtatt
      241 ttcagagtga ggtcaccaag aattccctgg agaaaatcct tccacagctg aaatgccatt
      301 tcacctggaa cttattcaag gaagacagtg tctcaaggga tctagaagat agagtgtgta
      361 accagattga atttttaaac actgagttca aagctacaat gtacaacttg ttggcctaca
      421 taaaacacct agatggtaac aacgaggcag ccctggaatg cttacggcaa gctgaagagt
      481 taatccagca agaacatgct gaccaagcag aaatcagaag tctagtcact tggggaaact
      541 acgcctgggt ctactatcac ttgggcagac tctcagatgc tcagatttat gtagataagg
      601 tgaaacaaac ctgcaagaaa ttttcaaatc catacagtat tgagtattct gaacttgact
      661 gtgaggaagg gtggacacaa ctgaagtgtg gaagaaatga aagggcgaag gtgtgttttg
      721 agaaggctct ggaagaaaag cccaacaacc cagaattctc ctctggactg gcaattgcga
      781 tgtaccatct ggataatcac ccagagaaac agttctctac tgatgttttg aagcaggcca
      841 ttgagctgag tcctgataac caatacgtca aggttctctt gggcctgaaa ctgcagaaga
      901 tgaataaaga agctgaagga gagcagtttg ttgaagaagc cttggaaaag tctccttgcc
      961 aaacagatgt cctccgcagt gcagccaaat tttacagaag aaaaggtgac ctagacaaag
     1021 ctattgaact gtttcaacgg gtgttggaat ccacaccaaa caatggctac ctctatcacc
     1081 agattgggtg ctgctacaag gcaaaagtaa gacaaatgca gaatacagga gaat
//
