LOCUS       sequence-86173-e        1099 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of IFIT5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Katibah GE, Lee HJ, Huizar JP, Vogan JM, Alber T, Collins K
  TITLE     tRNA binding, structure, and localization of the human 
            interferon-induced protein IFIT5.
  JOURNAL   Mol Cell. 2013 Feb 21;49(4):743-50. doi: 
            10.1016/j.molcel.2012.12.015. Epub 2013 Jan 11.
  PUBMED    23317505
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2012.12.015"; journalName: "Mol Cell"; date: 
            "2013-02-21- 21"; volume: "49"; issue: "4"; pages: "743-50"
COMMENT     Sequence Label: pET28a(+) 6xHis-TEV-IFIT5
FEATURES             Location/Qualifiers
     source          1..1099
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     69..88
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        69..87
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    88..112
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             127..149
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             168..185
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             195..212
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             228..248
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc
       61 cgcgaaatta atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga
      121 aataattttg tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc
      181 atcacagcag cggcctggtg ccgcgcggca gccatatggc tagcagagaa aacctgtatt
      241 ttcagagtga aattcgtaag gacaccttga aggccattct gttggagtta gaatgtcatt
      301 ttacatggaa tttacttaag gaagacattg atctgtttga ggtagaagat acaattgggc
      361 aacagcttga atttcttacc acaaaatcta gacttgctct ttataaccta ttggcctatg
      421 tgaaacacct aaaaggccaa aataaagacg cccttgagtg cttggaacaa gcagaagaaa
      481 taatccagca agaacactca gacaaagaag aagtacgaag cctggtcact tggggaaact
      541 atgcctgggt gtattatcac atggaccagc ttgaagaagc tcagaagtat acaggtaaga
      601 tagggaatgt ctgtaagaaa ttgtccagtc cttctaacta caagttggag tgtcctgaga
      661 ctgactgtga gaaaggctgg gcactcttga aatttggagg aaagtattat caaaaggcta
      721 aagcggcttt tgagaaggct ctggaagtgg agcctgacaa tccagaattt aacatcggct
      781 atgctatcac agtgtatcgg ctggatgatt ctgatagaga agggtctgta aagagctttt
      841 ctctggggcc tttgagaaag gctgttaccc tgaacccaga taacagctat attaaggttt
      901 ttctggcact gaagcttcaa gatgtacatg cagaagctga aggggaaaag tatattgaag
      961 aaatcctgga ccaaatatca tcccagcctt acgtccttcg ttatgcagcc aagttctata
     1021 ggagaaaaaa ttcctggaac aaagctctcg aacttttaaa aaaggccttg gaggtgacac
     1081 caacttcttc tttcctgca
//
