LOCUS       sequence-86206-e        1134 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of IFIT5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Katibah GE, Lee HJ, Huizar JP, Vogan JM, Alber T, Collins K
  TITLE     tRNA binding, structure, and localization of the human 
            interferon-induced protein IFIT5.
  JOURNAL   Mol Cell. 2013 Feb 21;49(4):743-50. doi: 
            10.1016/j.molcel.2012.12.015. Epub 2013 Jan 11.
  PUBMED    23317505
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2012.12.015"; journalName: "Mol Cell"; date: 
            "2013-02-21- 21"; volume: "49"; issue: "4"; pages: "743-50"
COMMENT     Sequence Label: pET28a(+) 6xHis-3xFlag-IFIT5
FEATURES             Location/Qualifiers
     source          1..1134
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     65..84
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        65..83
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    84..108
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             123..145
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             164..181
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             191..208
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             224..289
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             290..310
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg
       61 aaattaatac gactcactat aggggaattg tgagcggata acaattcccc tctagaaata
      121 attttgttta actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca
      181 cagcagcggc ctggtgccgc gcggcagcca tatggctagc agagactaca aagaccatga
      241 cggtgattat aaagatcatg acatcgatta caaggatgac gatgacaagg aaaacctgta
      301 ttttcagggc ggatccatga gtgaaattcg taaggacacc ttgaaggcca ttctgttgga
      361 gttagaatgt cattttacat ggaatttact taaggaagac attgatctgt ttgaggtaga
      421 agatacaatt gggcaacagc ttgaatttct taccacaaaa tctagacttg ctctttataa
      481 cctattggcc tatgtgaaac acctaaaagg ccaaaataaa gacgcccttg agtgcttgga
      541 acaagcagaa gaaataatcc agcaagaaca ctcagacaaa gaagaagtac gaagcctggt
      601 cacttgggga aactatgcct gggtgtatta tcacatggac cagcttgaag aagctcagaa
      661 gtatacaggt aagataggga atgtctgtaa gaaattgtcc agtccttcta actacaagtt
      721 ggagtgtcct gagactgact gtgagaaagg ctgggcactc ttgaaatttg gaggaaagta
      781 ttatcaaaag gctaaagcgg cttttgagaa ggctctggaa gtggagcctg acaatccaga
      841 atttaacatc ggctatgcta tcacagtgta tcggctggat gattctgata gagaagggtc
      901 tgtaaagagc ttttctctgg ggcctttgag aaaggctgtt accctgaacc cagataacag
      961 ctatattaag gtttttctgg cactgaagct tcaagatgta catgcagaag ctgaagggga
     1021 aaagtatatt gaagaaatcc tggaccaaat atcatcccag ccttacgtcc ttcgttatgc
     1081 agccaagttc tataggagaa aaaattcctg gaacaaagct ctcgaacttt taaa
//
