LOCUS       sequence-86279-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of human 6His-tagged SUMO2, SUMO-E1 and 
            SUMO-E2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Weber AR, Schuermann D, Schar P
  TITLE     Versatile Recombinant SUMOylation System for the Production of 
            SUMO-Modified Protein.
  JOURNAL   PLoS One. 2014 Jul 9;9(7):e102157. doi: 
            10.1371/journal.pone.0102157. eCollection 2014.
  PUBMED    25007328
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-9"; volume: "9(7):e102157. doi"; pages: " 
            10.1371/journal.pone.0102157. eCollection 2014"
COMMENT     Sequence Label: pSUMO2
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(55..102)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     98..116
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 attgactacc ggaagcagtg tgaccgtgtg cttctcaaat gcctgaggtt tcagcaaaaa
       61 acccctcaag acccgtttag aggccccaag gggttatgct agttattgct cagcggtggc
      121 agcagcctag cccttggggc caaggcactc cacaatccca ttccccttca tgccatcgaa
      181 gaagaagaag ttgttgtgag gagggtcccg ctgagacagg gccttcacaa tttcctgtgc
      241 caaaatccct ccaaccaccg cacacactgg ggccatctcg gagaagcagt acctgacaaa
      301 gtcctcagga agcaggtcag gactaatacc cagtgagtca agcacatcat ttcgtatctg
      361 gagcaacaac tcagaatctt cctcatatgt atcagaactg ggatctcttc ctttatctgt
      421 acggaacttt aagagcactt gaaggagaaa gtagtcggag gtcgtgcgct tcagagcagc
      481 ctttgctttc tcactgctcc agtccacctc cagggcttct ttaacagggc agaagaccac
      541 cttctttttg accatcgttg tctcagaaga atcaagtttt gctctcttgg tgtcgggccc
      601 atcttctact ccttggctaa ctttggcaac tttagttttc tcctctacaa actcatgctc
      661 tcctagattg gcaaatgtgt atccatggta gccaaaaaca tctcctgtaa agaacttgat
      721 gctatttttg tgacagatct ggtcaacttt aactatgaca tccctggagc agcaagtcag
      781 acacacagca tcgaattgag tgaaaaatga ctctggtttc ttctctatat cctcagtgtc
      841 caccttcaca tccaccatgg g
//
