LOCUS       sequence-86284-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of human 6His-tagged SUMO3, SUMO-E1 and 
            SUMO-E2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Weber AR, Schuermann D, Schar P
  TITLE     Versatile Recombinant SUMOylation System for the Production of 
            SUMO-Modified Protein.
  JOURNAL   PLoS One. 2014 Jul 9;9(7):e102157. doi: 
            10.1371/journal.pone.0102157. eCollection 2014.
  PUBMED    25007328
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-9"; volume: "9(7):e102157. doi"; pages: " 
            10.1371/journal.pone.0102157. eCollection 2014"
COMMENT     Sequence Label: pSUMO3
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(51..98)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     94..112
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 actaccggaa gcagtgtgac cgtgtgcttc tcaaatgcct gaggtttcag caaaaaaccc
       61 ctcaagaccc gtttagaggc cccaaggggt tatgctagtt attgctcagc ggtggcagca
      121 gcctagccct tggggccaag gcactccaca atcccattcc ccttcatgcc atcgaagaag
      181 aagaagttgt tgtgaggagg gtcccgctga gacagggcct tcacaatttc ctgtgccaaa
      241 atccctccaa ccaccgcaca cactggggcc atctcggaga agcagtacct gacaaagtcc
      301 tcaggaagca ggtcaggact aatacccagt gagtcaagca catcatttcg tatctggagc
      361 aacaactcag aatcttcctc atatgtatca gaactgggat ctcttccttt atctgtacgg
      421 aactttaaga gcacttgaag gagaaagtag tcggaggtcg tgcgcttcag agcagccttt
      481 gctttctcac tgctccagtc cacctccagg gcttctttaa cagggcagaa gaccaccttc
      541 tttttgacca tcgttgtctc agaagaatca agttttgctc tcttggtgtc gggcccatct
      601 tctactcctt ggctaacttt ggcaacttta gttttctcct ctacaaactc atgctctcct
      661 agattggcaa atgtgtatcc atggtagcca aaaacatctc ctgtaaagaa cttgatgcta
      721 tttttgtgac agatctggtc aactttaact atgacatccc tggagcagca agtcagacac
      781 acagcatcga attgagtgaa aaatgactct ggtttcttct ctatatcctc agtgtccacc
      841 ttcacatcca ccatggggtt gagattctga
//
