LOCUS       sequence-86287-e         689 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of human 6His-tagged SUMO1, SUMO-E1 and 
            SUMO-E2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Weber AR, Schuermann D, Schar P
  TITLE     Versatile Recombinant SUMOylation System for the Production of 
            SUMO-Modified Protein.
  JOURNAL   PLoS One. 2014 Jul 9;9(7):e102157. doi: 
            10.1371/journal.pone.0102157. eCollection 2014.
  PUBMED    25007328
REFERENCE   2  (bases 1 to 689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-9"; volume: "9(7):e102157. doi"; pages: " 
            10.1371/journal.pone.0102157. eCollection 2014"
COMMENT     Sequence Label: pSUMO1
FEATURES             Location/Qualifiers
     source          1..689
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(54..101)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     97..115
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggttt cagcaaaaaa
       61 cccctcaaga cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca
      121 gcagcctagc ccttggggcc aaggcactcc acaatcccat tccccttcat gccatcgaag
      181 aagaagaagt tgttgtgagg agggtcccgc tgagacaggg ccttcacaat ttcctgtgcc
      241 aaaatccctc caaccaccgc acacactggg gccatctcgg agaagcagta cctgacaaag
      301 tcctcaggaa gcaggtcagg actaataccc agtgagtcaa gcacatcatt tcgtatctgg
      361 agcaacaact cagaatcttc ctcatatgta tcagaactgg gatctcttcc tttatctgta
      421 cggaacttta agagcacttg aaggagaaag tagtcggagg tcgtgcgctt cagagcagcc
      481 tttgctttct cactgctcca gtccacctcc agggcttctt taacagggca gaagaccacc
      541 ttctttttga ccatcgttgt ctcagaagaa tcaagttttg ctctcttggt gtcgggccca
      601 tcttctactc cttggctaac tttggcaact ttagttttct cctctacaaa ctcatgctct
      661 cctagattgg caaatgtgta tccatggta
//
