LOCUS       sequence-86307-e         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of human TDG-K330A C-terminally fused to 
            SUMO-E2 and GST.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Weber AR, Schuermann D, Schar P
  TITLE     Versatile Recombinant SUMOylation System for the Production of 
            SUMO-Modified Protein.
  JOURNAL   PLoS One. 2014 Jul 9;9(7):e102157. doi: 
            10.1371/journal.pone.0102157. eCollection 2014.
  PUBMED    25007328
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-9"; volume: "9(7):e102157. doi"; pages: " 
            10.1371/journal.pone.0102157. eCollection 2014"
COMMENT     Sequence Label: pSC-hTDG-K330A-PreE2
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(517..540)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 aggggacatt ccggatcctg atgagggcgc aaacttcttg gcttgtgctc ggaccctttt
       61 ctcgtactcc actctgtttt ggcagtaaat cgtgtaggcc tctgcttgag ctgggtcttg
      121 gatatttggt tcatttagaa gttcctgtat tcctaatagg atctgtttga ttgtgatggc
      181 tggcctccag tccttgtcct cctctaagat ggacaggcac actgtccccg aagggtacac
      241 attcgggtga aataatggtg gttcgaattt acattttggt ggcgaagatg gataatcatc
      301 tttgaaaagc atccgtagtt taaacaagcc tccttcccac ggagtccctt tctttcccgg
      361 aatggcgcac tcccagttca tgaggttcat cgtgccatcg ggattttttg ttgggacagc
      421 cacgaaacca aatgggtggt ctttcctcca tgctttcctc tcctgggcga gtctgctgag
      481 ggcgatcccc gacatggatg ctaagcttcc gccacctgga ccctggaaca gaacctcgag
      541 gaattcagca tggctttctt cttcctgttc ttgtgttcca cagtgattac taaaggaagg
      601 aatttggtct gtaaatgact gggtcatcca ctgcccatta ggaatgccac tgaaagctga
      661 ttctcctctt aactccacgc tctcaattag cccatttgaa gagaagccac aaggttcact
      721 gctgcatgga ttttctccgt aagcaccacc atatgctgcc tcataacctg gatcatattt
      781 ttcttccgca acagccatct tctttgcatc ctcttggg
//
