LOCUS       sequence-86324-e        1089 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of TPP1 full length with 166-172 replaced with 
            ""GSSG"".
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Sexton AN, Youmans DT, Collins K
  TITLE     Specificity requirements for human telomere protein interaction with
            telomerase holoenzyme.
  JOURNAL   J Biol Chem. 2012 Oct 5;287(41):34455-64. doi: 
            10.1074/jbc.M112.394767. Epub 2012 Aug 14.
  PUBMED    22893708
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.394767"; journalName: "J Biol Chem"; date: 
            "2012-10-5- 5"; volume: "287"; issue: "41"; pages: "34455-64"
COMMENT     Sequence Label: pcDNA3.1 3xFLAG TPP1 88-544 delta166-172
FEATURES             Location/Qualifiers
     source          1..1089
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             102..167
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcat ggactacaaa gaccatgacg
      121 gtgattataa agatcatgac atcgattaca aggatgacga tgacaagaag cttgcaggtt
      181 cggggaggct ggtcctacgg ccctggattc gggagctgat tctggggtca gagacaccct
      241 ccagtccacg agccgggcag ctgcttgagg tactacagga cgccgaggcc gcggtcgcgg
      301 gcccatccca cgcccctgat acgtccgacg tcggggccac gctgcttgtg tctgacggga
      361 cccacagtgt ccgatgcctg gtgacgcggg aggccctgga cacctcggga agttcaggag
      421 gcttccgcgg gacagagggc cggctgctgc tgctgcagga ctgcggggtt catgtccagg
      481 tcgctgaggg cggcgcgccc gcagagttct atctccaggt ggaccgcttc agcctgctgc
      541 ccacggagca gccccggcta cgggtgcctg gttgcaacca agacttagat gttcagaaaa
      601 agctctatga ctgccttgag gagcaccttt cagagtccac ctcgtccaat gcaggcctat
      661 cactgtccca gcttctggat gaaatgcggg aggaccagga gcatcagggg gcactcgtgt
      721 gcctggctga aagctgcctg acactggagg gcccttgcac agcaccccct gtcacccact
      781 gggctgcctc acgatgcaag gccacgggag aagctgtgta cactgtcccc agctcaatgc
      841 tgtgcatctc tgagaatgac cagctaattc tgagctctct aggcccctgt cagaggacac
      901 agggccctga gctgccccca ccagacccgg ctctgcagga cctatctctg accctcatag
      961 cctctcctcc ttcctcaccc agttcctcag gaaccccggc cttacccggc cacatgtcat
     1021 ccgaggaaag tggtaccagc atcagccttc tgcctgccct gtccttggct gctccagacc
     1081 cagggcaga
//
