LOCUS       sequence-86328-e         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of human XRCC1-K176R C-terminally fused to 
            SUMO-E2 and GST.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Weber AR, Schuermann D, Schar P
  TITLE     Versatile Recombinant SUMOylation System for the Production of 
            SUMO-Modified Protein.
  JOURNAL   PLoS One. 2014 Jul 9;9(7):e102157. doi: 
            10.1371/journal.pone.0102157. eCollection 2014.
  PUBMED    25007328
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-9"; volume: "9(7):e102157. doi"; pages: " 
            10.1371/journal.pone.0102157. eCollection 2014"
COMMENT     Sequence Label: pSC-hXRCC1-K176R-PreE2
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(510..533)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 attccggatc ctgatgaggg cgcaaacttc ttggcttgtg ctcggaccct tttctcgtac
       61 tccactctgt tttggcagta aatcgtgtag gcctctgctt gagctgggtc ttggatattt
      121 ggttcattta gaagttcctg tattcctaat aggatctgtt tgattgtgat ggctggcctc
      181 cagtccttgt cctcctctaa gatggacagg cacactgtcc ccgaagggta cacattcggg
      241 tgaaataatg gtggttcgaa tttacatttt ggtggcgaag atggataatc atctttgaaa
      301 agcatccgta gtttaaacaa gcctccttcc cacggagtcc ctttctttcc cggaatggcg
      361 cactcccagt tcatgaggtt catcgtgcca tcgggatttt ttgttgggac agccacgaaa
      421 ccaaatgggt ggtctttcct ccatgctttc ctctcctggg cgagtctgct gagggcgatc
      481 cccgacatgg atgctaagct tccgccacct ggaccctgga acagaacctc gaggaattcg
      541 gcttgcggca ccaccccata gagctggtga ggaagtaact tctgcttctc attgcaactg
      601 tagatccatc ggggacgaac gaatgccagg gaggggttgt ccatcagggc ctcctcaaag
      661 ctgggatccc attcctgtgc tgtgatcaca aactgaaccc ggtcactcat atagtcctcg
      721 agctccccat tgaaggctgt gacgtatcgg atgagtttcc gccgctcgtc cccagggaac
      781 tccccgtaaa gaaa
//
