LOCUS       sequence-86351-e        1087 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of TPP1 full length.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Sexton AN, Youmans DT, Collins K
  TITLE     Specificity requirements for human telomere protein interaction with
            telomerase holoenzyme.
  JOURNAL   J Biol Chem. 2012 Oct 5;287(41):34455-64. doi: 
            10.1074/jbc.M112.394767. Epub 2012 Aug 14.
  PUBMED    22893708
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.394767"; journalName: "J Biol Chem"; date: 
            "2012-10-5- 5"; volume: "287"; issue: "41"; pages: "34455-64"
COMMENT     Sequence Label: pcDNA3.1 3xFLAG TPP1 88-544
FEATURES             Location/Qualifiers
     source          1..1087
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             102..167
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcat ggactacaaa gaccatgacg
      121 gtgattataa agatcatgac atcgattaca aggatgacga tgacaagaag cttgcaggtt
      181 cggggaggct ggtcctacgg ccctggattc gggagctgat tctggggtca gagacaccct
      241 ccagtccacg agccgggcag ctgcttgagg tactacagga cgccgaggcc gcggtcgcgg
      301 gcccatccca cgcccctgat acgtccgacg tcggggccac gctgcttgtg tctgacggga
      361 cccacagtgt ccgatgcctg gtgacgcggg aggccctgga cacctcggac tgggaggaga
      421 aggagttcgg cttccgcggg acagagggcc ggctgctgct gctgcaggac tgcggggttc
      481 atgtccaggt cgctgagggc ggcgcgcccg cagagttcta tctccaggtg gaccgcttca
      541 gcctgctgcc cacggagcag ccccggctac gggtgcctgg ttgcaaccaa gacttagatg
      601 ttcagaaaaa gctctatgac tgccttgagg agcacctttc agagtccacc tcgtccaatg
      661 caggcctatc actgtcccag cttctggatg aaatgcggga ggaccaggag catcaggggg
      721 cactcgtgtg cctggctgaa agctgcctga cactggaggg cccttgcaca gcaccccctg
      781 tcacccactg ggctgcctca cgatgcaagg ccacgggaga agctgtgtac actgtcccca
      841 gctcaatgct gtgcatctct gagaatgacc agctaattct gagctctcta ggcccctgtc
      901 agaggacaca gggccctgag ctgcccccac cagacccggc tctgcaggac ctatctctga
      961 ccctcatagc ctctcctcct tcctcaccca gttcctcagg aaccccggcc ttacccggcc
     1021 acatgtcatc cgaggaaagt ggtaccagca tcagccttct gcctgccctg tccttggctg
     1081 ctccaga
//
