LOCUS       sequence-86354-e        1015 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  9 copies of NFAT sites in minimal alphaMHC-pGL-3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Wilkins BJ, Dai YS, Bueno OF, Parsons SA, Xu J, Plank DM, Jones F, 
            Kimball TR, Molkentin JD
  TITLE     Calcineurin/NFAT coupling participates in pathological, but not 
            physiological, cardiac hypertrophy.
  JOURNAL   Circ Res. 2004 Jan 9;94(1):110-8. Epub 2003 Dec 1.
  PUBMED    14656927
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Circ Res.";
            date: "2004-01-9"; volume: "94(1)"; pages: "110-8. Epub 2003 Dec 1"
COMMENT     Sequence Label: 9X NFAT-apha-MHC-Luc
FEATURES             Location/Qualifiers
     source          1..1015
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      506..515
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(578..596)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 acatttctct atcgataggt accgagctct tacgcgtaca ttggaaaatt ttattacact
       61 acattggaaa attttattac actacattgg aaaattttat tacactacat tggaaaattt
      121 tattacacgc gtgctagcac attggaaaat tttattacac gctagccccg cgtacattgg
      181 aaaattttat tacactacat tggaaaattt tattacacta cattggaaaa ttttattaca
      241 ctacattgga aaattttatt acacgcgggg ctgctgtcct cctgtcacct ccagagccaa
      301 gggatcaaag gaggaggagc caggacagga gggatgggag ggagggtccc agcagatgac
      361 tccaaattta ggcagcaggc acgtggaatg agctataaag gggctggagc gctgagagct
      421 gtcagaccga gatttctcca tcccaagctt cggggctcga gatctgcgat ctaagtaagc
      481 ttggcattcc ggtactgttg gtaaagccac catggaagac gccaaaaaca taaagaaagg
      541 cccggcgcca ttctatccgc tggaagatgg aaccgctgga gagcaactgc ataaggctat
      601 gaagagatac gccctggttc ctggaacaat tgcttttaca gatgcacata tcgaggtgga
      661 catcacttac gctgagtact tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata
      721 tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc aattctttat
      781 gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg acatttataa
      841 tgaacgtgaa ttgctcaaca gtatgggcat ttcgcagcct accgtggtgt tcgtttccaa
      901 aaaggggttg caaaaaattt tgaacgtgca aaaaaagctc ccaatcatcc aaaaaattat
      961 tatcatggat tctaaaacgg attaccaggg atttcagtcg atgtacacgt tcgtc
//
