LOCUS       sequence-86355-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  destination vector with vacuole marker (gamma-TIP) - ECFP for tagged
            fluorescent protein (N-ter-EYFP) of plant gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Lao J, Oikawa A, Bromley JR, McInerney P, Suttangkakul A, 
            Smith-Moritz AM, Plahar H, Chiu TY, Gonzalez Fernandez-Nino SM, 
            Ebert B, Yang F, Christiansen KM, Hansen SF, Stonebloom S, Adams PD,
            Ronald PC, Hillson NJ, Hadi MZ, Vega-Sanchez ME, Loque D, Scheller 
            HV, Heazlewood1 JL
  TITLE     The Plant Glycosyltransferase Clone Collection for Functional 
            Genomics.
  JOURNAL   Plant J. 2014 Jun 6. doi: 10.1111/tpj.12577.
  PUBMED    24905498
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2014 Jun 6. doi: 10.1111/tpj.12577."
COMMENT     Sequence Label: pBullet-vac-n
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(18..39)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(279..298)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             438..689
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     626..647
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    696..820
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
       61 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      121 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctgc
      181 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
      241 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
      301 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
      361 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
      421 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
      481 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
      541 gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca
      601 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
      661 tcactctcgg catggacgag ctgtacaagc atatgacaag tttgtacaaa aaagctgaac
      721 gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga
      781 ctacataata ctgtaaaaca caacatatcc agtcactatg aatcaac
//
