LOCUS       sequence-86363-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of human XRCC1-K176R with N-terminal GST-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Weber AR, Schuermann D, Schar P
  TITLE     Versatile Recombinant SUMOylation System for the Production of 
            SUMO-Modified Protein.
  JOURNAL   PLoS One. 2014 Jul 9;9(7):e102157. doi: 
            10.1371/journal.pone.0102157. eCollection 2014.
  PUBMED    25007328
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-9"; volume: "9(7):e102157. doi"; pages: " 
            10.1371/journal.pone.0102157. eCollection 2014"
COMMENT     Sequence Label: pGEX-hXRCC1-K176R
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    38..54
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(62..68,69..84,85..90))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     CDS             complement(192..719)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGISLSTARCTNASGVR
                     QPSEAVVWLCRS"
     primer_bind     668..690
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     682..699
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     683..699
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(715..731)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(715..731)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    739..755
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(763..769,770..787,788..793))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 tagtataggg gacatgaata ctgtttcctg tgtgaaattg ttatccgctc acaattccac
       61 acattatacg agccgatgat taattgtcaa cagctcattt cagaatattt gccagaaccg
      121 ttatgatgtc ggcgcaaaaa acattatcca gaacgggagt gcgccttgag cgacacgaat
      181 tatgcagtga tttacgacct gcacagccat accacagctt ccgatggctg cctgacgcca
      241 gaagcattgg tgcaccgtgc agtcgataag ctaattccaa cgccatcaaa aataattcgc
      301 gtctggcctt cctgtagcca gctttcatca acattaaatg tgagcgagta acaacccgtc
      361 ggattctccg tgggaacaaa cggcggattg accgtaatgg gataggtcac gttggtgtag
      421 atgggcgcat cgtaaccgtg catctgccag tttgagggga cgacgacagt atcggcctca
      481 ggaagatcgc actccagcca gctttccggc accgcttctg gtgccggaaa ccaggcaaag
      541 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
      601 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
      661 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aatccgtaat catggtcata
      721 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
      781 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg
      841 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
      901 acgcgcgggg agaggcggtt tgcgtattgg
//
