LOCUS       sequence-86364-e         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Adenoviral vector for dominant negative JNK1 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Liang Q, Bueno OF, Wilkins BJ, Kuan CY, Xia Y, Molkentin JD
  TITLE     c-Jun N-terminal kinases (JNK) antagonize cardiac growth through 
            cross-talk with calcineurin-NFAT signaling.
  JOURNAL   EMBO J. 2003 Oct 1;22(19):5079-89.
  PUBMED    14517246
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2003-10-1"; volume: "22(19)"; pages: "5079-89"
COMMENT     Sequence Label: pLpA-DN.JNK1
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..30
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    40..96
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             111..134
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agcagagctc gtttagtgaa ccgtcagatc cggtcgcgcg aattcgagct cggtacccgg
       61 ggatcctcta gagtcgacct gcaggcatgc aagcttcggc agccaacatg gactataagg
      121 acgatgatga caaaagcaga agcaagcgtg acaacaattt ttatagtgta gagattggag
      181 attctacatt cacagtcctg aaacgatatc agaatttaaa acctataggc tcaggagctc
      241 aaggaatagt atgcgcagct tatgatgcca ttcttgaaag aaatgttgca atcaagaagc
      301 taagccgacc atttcagaat cagactcatg ccaagcgggc ctacagagag ctagttctta
      361 tgaaatgtgt taatcacaaa aatataattg gccttttgaa tgttttcaca ccacagaaat
      421 ccctagaaga atttcaagat gtttacatag tcatggagct catggatgca aatctttgcc
      481 aagtgattca gatggagcta gatcatgaaa gaatgtccta ccttctctat cagatgctgt
      541 gtggaatcaa gcaccttcat tctgctggaa ttattcatcg ggacttaaag cccagtaata
      601 tagtagtaaa atctgattgc actttgaaga ttcttgactt cggtctggcc aggactgcag
      661 gaacgagttt tatgatggcg ccttttgtcg tcacacggta ttacagagca cccgaggtca
      721 tccttggcat gggctacaag gaaaacgtgg atttatggtc tgtggggtgc attatgggag
      781 aaatggtttg ccacaaaatc ctctttccag gaagggacta tattgatcag tggaataaag
      841 ttattgaaca gcttggaaca ccatgtcctg aattcatgaa gaaactgcaa ccaacagtaa
      901 ggacttacgt tgaaaacaga cctaaatatg ctggatatag ctttgagaaa ctcttccctg
      961 atgtcctttt cccagctgac tcagaaacac a
//
