LOCUS       sequence-86369-e        1075 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of TPP1 OB-fold domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Sexton AN, Youmans DT, Collins K
  TITLE     Specificity requirements for human telomere protein interaction with
            telomerase holoenzyme.
  JOURNAL   J Biol Chem. 2012 Oct 5;287(41):34455-64. doi: 
            10.1074/jbc.M112.394767. Epub 2012 Aug 14.
  PUBMED    22893708
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.394767"; journalName: "J Biol Chem"; date: 
            "2012-10-5- 5"; volume: "287"; issue: "41"; pages: "34455-64"
COMMENT     Sequence Label: pcDNA3.1 3xFLAG TPP1 88-249
FEATURES             Location/Qualifiers
     source          1..1075
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             102..167
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(700..717)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    706..930
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcat ggactacaaa gaccatgacg
      121 gtgattataa agatcatgac atcgattaca aggatgacga tgacaagaag cttgcaggtt
      181 cggggaggct ggtcctacgg ccctggattc gggagctgat tctggggtca gagacaccct
      241 ccagtccacg agccgggcag ctgcttgagg tactacagga cgccgaggcc gcggtcgcgg
      301 gcccatccca cgcccctgat acgtccgacg tcggggccac gctgcttgtg tctgacggga
      361 cccacagtgt ccgatgcctg gtgacgcggg aggccctgga cacctcggac tgggaggaga
      421 aggagttcgg cttccgcggg acagagggcc ggctgctgct gctgcaggac tgcggggttc
      481 atgtccaggt cgctgagggc ggcgcgcccg cagagttcta tctccaggtg gaccgcttca
      541 gcctgctgcc cacggagcag ccccggctac gggtgcctgg ttgcaaccaa gacttagatg
      601 ttcagaaaaa gctctatgac tgccttgagg agcacctttc agagtccacc tcgtccaatt
      661 gactcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
      721 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
      781 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
      841 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
      901 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
      961 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     1021 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gcttt
//
