LOCUS       sequence-86372-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  destination vector with PIP2a-ECFP (plasma membrane marker) for 
            tagged fluorescent protein (N-ter-EYFP) of plant gene .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Lao J, Oikawa A, Bromley JR, McInerney P, Suttangkakul A, 
            Smith-Moritz AM, Plahar H, Chiu TY, Gonzalez Fernandez-Nino SM, 
            Ebert B, Yang F, Christiansen KM, Hansen SF, Stonebloom S, Adams PD,
            Ronald PC, Hillson NJ, Hadi MZ, Vega-Sanchez ME, Loque D, Scheller 
            HV, Heazlewood1 JL
  TITLE     The Plant Glycosyltransferase Clone Collection for Functional 
            Genomics.
  JOURNAL   Plant J. 2014 Jun 6. doi: 10.1111/tpj.12577.
  PUBMED    24905498
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2014 Jun 6. doi: 10.1111/tpj.12577."
COMMENT     Sequence Label: pBullet-pm-n
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(25..46)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(join(181..693,694..696,697..699))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     374..393
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     633..654
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gccgagagtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc
       61 gttggggtct ttgctcaggg cggactgggt gctcaggtag tggttgtcgg gcagcagcac
      121 ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc
      181 ctcgatgttg tggcggatct tgaagttggc cttgatgccg ttcttctgct tgtcggcggt
      241 gatatagacg ttgtggctga tgtagttgta ctccagcttg tgccccagga tgttgccgtc
      301 ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt
      361 cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac
      421 gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg
      481 gctgaagcac tgcacgcccc aggtcagggt ggtcacgagg gtgggccagg gcacgggcag
      541 cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc
      601 ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg
      661 caccaccccg gtgaacagct cctcgccctt gctcaccatg gatcccccgg gcacgacgtt
      721 ggcagcactt ctgaatgatc caagagactt agaacctgaa gctcttagaa cgaattggtg
//
