LOCUS       sequence-86373-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of TARGET proteins C-terminally fused to GyrA 
            intein and GST.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Weber AR, Schuermann D, Schar P
  TITLE     Versatile Recombinant SUMOylation System for the Production of 
            SUMO-Modified Protein.
  JOURNAL   PLoS One. 2014 Jul 9;9(7):e102157. doi: 
            10.1371/journal.pone.0102157. eCollection 2014.
  PUBMED    25007328
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-9"; volume: "9(7):e102157. doi"; pages: " 
            10.1371/journal.pone.0102157. eCollection 2014"
COMMENT     Sequence Label: pTXG
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..623)
                     /codon_start=1
                     /gene="Mycobacterium xenopi gyrA"
                     /product="modified GyrA intein (Southworth et al., 1999)"
                     /label=Mxe GyrA intein
                     /note="N-terminal cleavage of this intein can be induced by
                     thiols."
                     /translation="CICGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPV
                     LADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAV
                     IQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYY
                     AKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHA"
     RBS             676..698
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    713..737
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(737..756)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(738..756)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aggcctgagt tcagaccggt gaggccagta gcgtggctga cgaacccgtt cgtgataaac
       61 gcgtggtctg ccgtgtcgac acgaaggcta tacaccggct gcacgccggc gtcggtgaca
      121 ctggcgactt tcgcgtagta gaaccgcccg tcggtcagct cgtcggcgat agcttgggcg
      181 tccgggtctc ggtggtgtgc ttccaagaaa cgcaccagtc cagggacgcc gactgtgtag
      241 gttgtgggcg caaattcggg tttcccgcgg gcaaaacctg cacagtcgac gctgaatgcg
      301 ctgcgttgaa tcaccgcgta atcgcccggc ttgatttcgt cgatcagctt ccacagcagg
      361 gtcggcaccc cggcgacgtc gaccaaacac aacaacgggt ggttcgcggt gcccgtcaca
      421 cgcagacctt cgaccgtacg caccgtgtac accggatgct cgccggagtg gaacagccgg
      481 tcggcgagca cgggattgcc atgccggtca aggactttca ggtcgatggc gttgtcactg
      541 ttgggccgcg cacccggcac gatgtcggcg atgcgtaccg actcgccctc gggtagggca
      601 actagtgcat ctccgcagat gcacatctcg aggaattcgc ggccgccgtc gactcgcgag
      661 ctagccatgg ttatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt
      721 tatccgctca caattcccct atagtgagtc gtattaattt cgcgggatcg agatccccgg
      781 tttaaaccgg gaattaattc cgatccccaa ttcctggcag tttatggcgg gcgtcctgcc
      841 cgccaccctc cgggccgttg cttcgcaa
//
