LOCUS       sequence-86381-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  destination vector with PIP2a-ECFP (plasma membrane marker) for 
            tagged fluorescent protein (C-ter-EYFP) of plant gene .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Lao J, Oikawa A, Bromley JR, McInerney P, Suttangkakul A, 
            Smith-Moritz AM, Plahar H, Chiu TY, Gonzalez Fernandez-Nino SM, 
            Ebert B, Yang F, Christiansen KM, Hansen SF, Stonebloom S, Adams PD,
            Ronald PC, Hillson NJ, Hadi MZ, Vega-Sanchez ME, Loque D, Scheller 
            HV, Heazlewood1 JL
  TITLE     The Plant Glycosyltransferase Clone Collection for Functional 
            Genomics.
  JOURNAL   Plant J. 2014 Jun 6. doi: 10.1111/tpj.12577.
  PUBMED    24905498
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2014 Jun 6. doi: 10.1111/tpj.12577."
COMMENT     Sequence Label: pBullet-pm-c
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(497..621)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(655..671)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(656..672)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(675..721)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(710..730)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg
       61 gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgatac agtagaaatt
      121 acagaaactt tatcacgttt agtaagtata gaggctgaaa atccagatga agccgaacga
      181 cttgtaagag aaaagtataa gagttgtgaa attgttcttg atgcagatga ttttcaggac
      241 tatgacacta gcgtatatga ataggtagat gtttttattt tgtcacacaa aaaagaggct
      301 cgcacctctt tttcttattt ctttttatga tttaatacgg cattgaggac aatagcgagt
      361 aggctggata cgacgattcc gtttgagaag aacatttgga aggctgtcgg tcgactaagt
      421 tggcagcatc acccgaagaa catttggaag gctgtcggtc gactacaggt cactaatacc
      481 atctaagtag ttgattcata gtgactggat atgttgtgtt ttacagtatt atgtagtctg
      541 ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc
      601 agcttttttg tacaaacttg ttgatgggct gcaggaattc gatatcaagc ttatcgatac
      661 cgtcgacctc gaggtcctct ccaaatgaaa tgaacttcct tatatagagg aagggtcttg
      721 cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatatcac atcaatccac
//
