LOCUS       sequence-86401-e        1035 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of TPP1 OB-fold domain with 166-172 replaced 
            with ""GSSG"".
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Sexton AN, Youmans DT, Collins K
  TITLE     Specificity requirements for human telomere protein interaction with
            telomerase holoenzyme.
  JOURNAL   J Biol Chem. 2012 Oct 5;287(41):34455-64. doi: 
            10.1074/jbc.M112.394767. Epub 2012 Aug 14.
  PUBMED    22893708
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.394767"; journalName: "J Biol Chem"; date: 
            "2012-10-5- 5"; volume: "287"; issue: "41"; pages: "34455-64"
COMMENT     Sequence Label: pcDNA3.1 3xFLAG TPP1 88-249 delta166-172
FEATURES             Location/Qualifiers
     source          1..1035
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             102..167
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(691..708)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    697..921
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcat ggactacaaa gaccatgacg
      121 gtgattataa agatcatgac atcgattaca aggatgacga tgacaagaag cttgcaggtt
      181 cggggaggct ggtcctacgg ccctggattc gggagctgat tctggggtca gagacaccct
      241 ccagtccacg agccgggcag ctgcttgagg tactacagga cgccgaggcc gcggtcgcgg
      301 gcccatccca cgcccctgat acgtccgacg tcggggccac gctgcttgtg tctgacggga
      361 cccacagtgt ccgatgcctg gtgacgcggg aggccctgga cacctcggga agttcaggag
      421 gcttccgcgg gacagagggc cggctgctgc tgctgcagga ctgcggggtt catgtccagg
      481 tcgctgaggg cggcgcgccc gcagagttct atctccaggt ggaccgcttc agcctgctgc
      541 ccacggagca gccccggcta cgggtgcctg gttgcaacca agacttagat gttcagaaaa
      601 agctctatga ctgccttgag gagcaccttt cagagtccac ctcgtccaat tgactcgagt
      661 ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
      721 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
      781 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
      841 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
      901 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
      961 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     1021 tgaccgctac acttg
//
