LOCUS       sequence-86419-e         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Cheeseman IM, Desai A
  TITLE     A combined approach for the localization and tandem affinity 
            purification of protein complexes from metazoans.
  JOURNAL   Sci STKE. 2005 Jan 11;2005(266):pl1.
  PUBMED    15644491
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci STKE.";
            date: "2005-01-11"; volume: "2005(266)"; pages: "pl1"
COMMENT     Sequence Label: pIC242
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(3..213)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9..144
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(14..311)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     309..329
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aaagcctagg cctccaaaaa agcctcctca ctacttctgg aatagctcag aggccgaggc
       61 ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag aatgggcgga
      121 actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg ttgctgacta
      181 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacacct
      241 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga
      301 ctttccacac cctaactgac acacattcca cagggtcgac cactgtgctg gcgaattgca
      361 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
      421 tctacaaatg tggtatggct gattatgatc agttatctag atccggtgga tcccgggccc
      481 gcggtaccgt cgactgcaga attcgaagct tttatctgtc tttagaccta agtttctcta
      541 ttaattcttg aagaggtgcc agctcacgcc tatcaatcag attaaactcc tgaacaaaga
      601 aaataaagtg cttaaaggag gtgttgaggt gggcctcctc ttgcagctgc atcacagaat
      661 caaagtgctg gtgataaata tgggcataaa ccctgaacag acgctttaga atagtctttg
      721 ccacagacat aaagtttttg ggaaatggga caccaatctt agaaggaaaa agagtttcat
      781 catcaagctg atcttgaacc caagtcatca aatagtcaat gtattttggt gcagaacatt
      841 tgattggctt tttaatatta gtaccatctg cccagtgata ttcatatctc ggacctgcag
      901 acatgactgg acagcttgct tcagtgca
//
