LOCUS       sequence-86425-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  destination vector with WAK2 (29 aa)-ECFP- ER marker (HDEL) for 
            tagged fluorescent protein (C-ter-EYFP) of plant gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Lao J, Oikawa A, Bromley JR, McInerney P, Suttangkakul A, 
            Smith-Moritz AM, Plahar H, Chiu TY, Gonzalez Fernandez-Nino SM, 
            Ebert B, Yang F, Christiansen KM, Hansen SF, Stonebloom S, Adams PD,
            Ronald PC, Hillson NJ, Hadi MZ, Vega-Sanchez ME, Loque D, Scheller 
            HV, Heazlewood1 JL
  TITLE     The Plant Glycosyltransferase Clone Collection for Functional 
            Genomics.
  JOURNAL   Plant J. 2014 Jun 6. doi: 10.1111/tpj.12577.
  PUBMED    24905498
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2014 Jun 6. doi: 10.1111/tpj.12577."
COMMENT     Sequence Label: pBullet-er-c
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(12..722,723..725,726..728))
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(54..75)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     403..422
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     662..683
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tgagatctct tcttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac
       61 tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactgggtg
      121 ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg
      181 tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttggcc
      241 ttgatgccgt tcttctgctt gtcggcggtg atatagacgt tgtggctgat gtagttgtac
      301 tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg
      361 cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg
      421 tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag
      481 tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact gcacgcccca ggtcagggtg
      541 gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc
      601 agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt
      661 acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg
      721 ctcaccatgg atccgcactc cttgcgaggt tgccccttga ctagctgcgt ataagcaagg
      781 tagaaaacag ccaccacgaa caaaccctcc tgtaccttca t
//
