LOCUS       sequence-86435-e         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  destination vector with ECFP-trans golgi network marker (VTI12) for 
            tagged fluorescent protein (N-ter-EYFP) of plant gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Lao J, Oikawa A, Bromley JR, McInerney P, Suttangkakul A, 
            Smith-Moritz AM, Plahar H, Chiu TY, Gonzalez Fernandez-Nino SM, 
            Ebert B, Yang F, Christiansen KM, Hansen SF, Stonebloom S, Adams PD,
            Ronald PC, Hillson NJ, Hadi MZ, Vega-Sanchez ME, Loque D, Scheller 
            HV, Heazlewood1 JL
  TITLE     The Plant Glycosyltransferase Clone Collection for Functional 
            Genomics.
  JOURNAL   Plant J. 2014 Jun 6. doi: 10.1111/tpj.12577.
  PUBMED    24905498
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2014 Jun 6. doi: 10.1111/tpj.12577."
COMMENT     Sequence Label: pBullet-tgn-n
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(496..620)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(627..878)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(669..690)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg
       61 aatataaatg tcaggctccc ttatacacag ccagtctgca ggtcgataca gtagaaatta
      121 cagaaacttt atcacgttta gtaagtatag aggctgaaaa tccagatgaa gccgaacgac
      181 ttgtaagaga aaagtataag agttgtgaaa ttgttcttga tgcagatgat tttcaggact
      241 atgacactag cgtatatgaa taggtagatg tttttatttt gtcacacaaa aaagaggctc
      301 gcacctcttt ttcttatttc tttttatgat ttaatacggc attgaggaca atagcgagta
      361 ggctggatac gacgattccg tttgagaaga acatttggaa ggctgtcggt cgactaagtt
      421 ggcagcatca cccgaagaac atttggaagg ctgtcggtcg actacaggtc actaatacca
      481 tctaagtagt tgattcatag tgactggata tgttgtgttt tacagtatta tgtagtctgt
      541 tttttatgca aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca
      601 gcttttttgt acaaacttgt catatgcttg tacagctcgt ccatgccgag agtgatcccg
      661 gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc
      721 agggcggact ggtagctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg
      781 ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg
      841 atcttgaagt tcaccttgat gccgttcttc tgcttgtcg
//
