LOCUS       sequence-86439-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  destination vector with ECFP-trans golgi network marker (VTI12) for 
            tagged fluorescent protein (N-ter-EYFP) of plant gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Lao J, Oikawa A, Bromley JR, McInerney P, Suttangkakul A, 
            Smith-Moritz AM, Plahar H, Chiu TY, Gonzalez Fernandez-Nino SM, 
            Ebert B, Yang F, Christiansen KM, Hansen SF, Stonebloom S, Adams PD,
            Ronald PC, Hillson NJ, Hadi MZ, Vega-Sanchez ME, Loque D, Scheller 
            HV, Heazlewood1 JL
  TITLE     The Plant Glycosyltransferase Clone Collection for Functional 
            Genomics.
  JOURNAL   Plant J. 2014 Jun 6. doi: 10.1111/tpj.12577.
  PUBMED    24905498
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2014 Jun 6. doi: 10.1111/tpj.12577."
COMMENT     Sequence Label: pBullet-tgn-n
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        431..776
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     719..739
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     complement(842..863)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gcgcgttggc cgattcatta atgcggccgc gagctcccta cccctactcc aaaaatgtca
       61 aagatacagt ctcagaagac caaagggcta ttgagacttt tcaacaaagg gtaatttcgg
      121 gaaacctcct cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa
      181 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggctatc attcaagatg
      241 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
      301 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgacatctcc actgacgtaa
      361 gggatgacgc acaatcccac ccctactcca aaaatgtcaa agatacagtc tcagaagacc
      421 aaagggctat tgagactttt caacaaaggg taatttcggg aaacctcctc ggattccatt
      481 gcccagctat ctgtcacttc atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat
      541 gccatcattg cgataaagga aaggctatca ttcaagatgc ctctgccgac agtggtccca
      601 aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt
      661 caaagcaagt ggattgatgt gacatctcca ctgacgtaag ggatgacgca caatcccact
      721 atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag aggacagccc
      781 agatctttgt ttgggaatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
      841 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtc
//
