LOCUS       sequence-86446-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E.coli expression of full length human Aos1 (SAE1) with Nterminal 
            His-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Pichler A, Gast A, Seeler JS, Dejean A, Melchior F
  TITLE     The nucleoporin RanBP2 has SUMO1 E3 ligase activity.
  JOURNAL   Cell. 2002 Jan 11;108(1):109-20.
  PUBMED    11792325
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2002-01-11"; volume: "108(1)"; pages: "109-20"
COMMENT     Sequence Label: pET28a-His-hAos1
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    70..94
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             109..131
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             150..167
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             177..194
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat taatacgact
       61 cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt
      121 taagaaggag atataccatg ggcagcagcc atcatcatca tcatcacagc agcggcctgg
      181 tgccgcgcgg cagccatatg gctagcatgg tggagaagga ggaggctggc ggcggcatta
      241 gcgaggagga ggcggcacag tatgaccggc agatccgcct gtggggactg gaggcccaga
      301 aacggctgcg ggcctctcgg gtgcttcttg tcggcttgaa aggacttggg gctgaaattg
      361 ccaagaatct catcttggca ggagtgaaag gactgaccat gctggatcac gaacaggtaa
      421 ctccagaaga tcccggagct cagttcttga ttcgtactgg gtctgttggc cgaaataggg
      481 ctgaagcctc tttggagcga gctcagaatc tcaaccccat ggtggatgtg aaggtggaca
      541 ctgaggatat agagaagaaa ccagagtcat ttttcactca attcgatgct gtgtgtctga
      601 cttgctgctc cagggatgtc atagttaaag ttgaccagat ctgtcacaaa aatagcatca
      661 agttctttac aggagatgtt tttggctacc atggatacac atttgccaat ctaggagagc
      721 atgagtttgt agaggagaaa actaaagttg ccaaagttag ccaaggagta gaagatgggc
      781 ccgacaccaa gagagcaaaa cttgattctt ctgagacaac gatggtcaaa
//
