LOCUS       sequence-86464-e         592 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Stelnicki EJ, Koemuves LG, Holmes D, Clavin W, Harrison MR, Adzick 
            NS, Largman C
  TITLE     The human homeobox genes MSX-1, MSX-2, and MOX-1 are differentially 
            expressed in the dermis and epidermis in fetal and adult skin.
  JOURNAL   Differentiation. 1997 Oct . 62(1):33-41.
  PUBMED    9373945
REFERENCE   2  (bases 1 to 592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Differentiation. 1997 Oct . 62(1):33-41."
COMMENT     Sequence Label: MSX1-pET
FEATURES             Location/Qualifiers
     source          1..592
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..78
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             82..114
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc
       61 ctggtgccgc gcggcagcca tatggctagc atgactggtg gacagcaaat gggtcgcgga
      121 tctcgagctg cggctggagg gggggtccgg ctctgcatgg ccccggctgc tgctatgact
      181 tctttgccac tcggtgtcaa agtggaggac tccgccttcg ccaagcctgc tgggggaggc
      241 gttggccaag cccccggggc tgctgcggcc accgcaaccg ccatgggcac agatgaggag
      301 ggggccaagc ccaaagtgcc cgcttcactc ctgcccttca gcgtggaggc cctcatggcc
      361 gatcacagga agcccggggc caaggagagc gtcctggtgg cctccgaagg ggctcaggca
      421 gcgggtggct cggtgcagca cttgggcacc cggcccgggt ctctgggcgc cccggatgcg
      481 ccctcctcgc cgcggcctct cggccatttc tcagtcggag gactcctcaa gctgccagaa
      541 gatgctctgg tgaaggccga aagccccgag aaactagatc ggaccccgtg ga
//
