LOCUS       sequence-86465-e         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid can be used to mutate genes in Toxoplasma gondii using 
            the CRISPR/Cas9 system after having an appropriate protospacer 
            cloned into it..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  TITLE     Lourido lab Toxoplasma CRISPR Plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pU6-Universal
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(6..53)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 tcttactttt tcttttttgc ctggccggcc tttttcgtgg ccgccggcct tttgtcgcct
       61 cccagctgag acaggtcgat ccgtgtctcg tacaggccgg tgatgctctg gtggatcagg
      121 gtggcgtcca gcacctcttt ggtgctggtg tacctcttcc ggtcgatggt ggtgtcaaag
      181 tacttgaagg cggcaggggc tcccagattg gtcagggtaa acaggtggat gatattctcg
      241 gcctgctctc tgatgggctt atcccggtgc ttgttgtagg cggacagcac tttgtccaga
      301 ttagcgtcgg ccaggatcac tctcttggag aactcgctga tctgctcgat gatctcgtcc
      361 aggtagtgct tgtgctgttc cacaaacagc tgtttctgct cattatcctc gggggagccc
      421 ttcagcttct catagtggct ggccaggtac aggaagttca catatttgga gggcagggcc
      481 agttcgtttc ccttctgcag ttcgccggca gaggccagca ttctcttccg gccgttttcc
      541 agctcgaaca gggagtactt aggcagcttg atgatcaggt cctttttcac ttctttgtag
      601 cccttggctt ccagaaagtc gatgggattc ttctcgaagc tgcttctttc catgatggtg
      661 atccccagca gctctttcac actcttcagt ttcttggact tgcccttttc cactttggcc
      721 accaccagca cagaataggc cacggtgggg ctgtcgaagc cgccgtactt cttagggtcc
      781 cagtccttct ttctggcgat cagcttatcg ctgttcctct tgggcaggat agactctttg
      841 ctgaagccgc ctgtctgcac ctcggtcttt ttcacgatat tcacttgggg catgctcagc
      901 actttccgca cggtgg
//
